维甲酸对SK-N-SH细胞增殖和相关基因表达的影响  

The Effect of Retinoic Acid on Proliferation and Associated Gene Expression of SK-N-SH Cell

作　　者：张秀艳[1] 梁盈[1] 李祺福[1] 宋建晔[1] 荆光军[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学和肿瘤细胞工程教育部重点实验室,福建厦门361005

出　　处：《现代生物医学进展》2008年第7期1201-1204,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金(No.30470877)

摘　　要：目的:研究维甲酸对SK-N-SH细胞的增殖和相关基因表达的影响。方法:通过苔盼蓝排除法绘制细胞生长曲线、流式细胞技术测定细胞周期、HE染色光镜观察细胞形态改变以及SABC免疫细胞化学染色法观察维甲酸对SK-N-SH相关癌基因、抑癌基因表达的影响。结果:1μmol/L RA处理后,SK-N-SH细胞的增殖活动受到明显的抑制,处理第7天抑制率达36.16%;周期测定显示处理后出现明显的G0/G1期阻滞,由对照组的49.7%增加至62.7%,增加了26.2%;免疫细胞化学染色结果显示,处理后细胞癌基因c-myc、c-fos的表达较对照组明显降低,而抑癌基因p53、p27的表达则有所加强。结论:维甲酸能有效抑制SK-N-SH细胞的增殖活动,其对细胞的增殖抑制作用与RA下调c-myc、c-fos等癌基因以及上调p53、p27等抑癌基因的表达有关。Objective： To study the effect of retinoic acid on proliferation and associated gene expression of SK-N-SH cell. Methods： Effects of retinoic acid on proliferation of SK-N-SH cells were determined with Taipan-blue dye exclusion test, effects on cell cycle were detected by flow cytometry, effects on morphology were detected by HE staining. And expression levels of oncogene and tumor suppressor gene were examined by immunocytochemistry. Results： The experiment results showed that after treaed with 1 μmol/L RA,the proliferation rate of SK-N-SH cells was inhibited, the inhitbion rate amounts to 36.16 %, and the cell cycle was arrested at G0/G1 phase, it was increased from 49.7 % to 62.7 %. The immunocytochemistry assay also revealed that the expression level of oncogene including c-myc c-fos was downregulated, and the expression level of tumor suppressor gene including p53 and p27 was upregulated. Conclusions： The results showed that the RA could effectively inhibit the proliferation activity of the SK-N-SH cell, and the mechanism of RA was related to its downregulating the expression levels of c-myc c-fos and upregulating the expression levels of p53 and p27.

关 键 词：神经母细胞瘤SK-N—SH细胞 维甲酸 细胞分化 基因表达 

分 类 号：Q247[生物学—细胞生物学] Q279