一株产碱性蛋白酶的嗜碱芽孢杆菌的分离和鉴定  

Isolation and Identification of Bacillus Alcalophilus with Alkaline Protease Activity

作　　者：孙同毅[1] 邵伟光[2] 高志芹[1] 林维平[1] 路福平[3] 于小勇[2] 王新斌 

机构地区：[1]山东潍坊医学院基础教学部,潍坊261042 [2]山东潍坊医学院附属医院影像中心,潍坊261031 [3]天津市工业微生物重点实验室,天津科技大学生物工程学院,天津300457 [4]山东潍坊坊子区中医院放射科,潍坊261200

出　　处：《现代生物医学进展》2008年第7期1256-1258,共3页Progress in Modern Biomedicine

基　　金：国家高技术研究发展计划(863计划)(2007AA02Z212);国家科技资源平台项目(2005DKA21204-10)

摘　　要：从天津塘沽盐碱土壤中分离一株产碱性蛋白酶的嗜碱芽孢杆菌HAP，并对其进行了表型分类和16S rDNA序列分析。结果表明，HAP是一株革兰氏阳性芽孢杆菌，菌体大小（0．7—0．9）μm×（2-3）μm，该菌可利用木糖、葡萄糖、阿拉伯糖、甘露醇，发酵型糖代谢产酸；可以水解酪素、淀粉，但不能水解酪氨酸；能够利用柠檬酸盐；其酶活力为1．22×10^4U／mL。结合系统发育学分析将HAP鉴定为嗜碱芽孢杆菌（Bacillus alcalophilus）。An isolate from alkali soil of Tanggu, Tianjin with alkaline protease activity was identified by phenotypic classification and phylogenetic analysis. The results show that the strain HAP is a gram-postive bacillus with about （0.7-0.9） μm×（2-3） μm width×length. Many kinds of carbon sources and nitrogen sources could be utilized in the medium. Physiological and biochemical test showed that xylose, glucose, arabinose and mannose can be utilized by the strain as fermentable sugar for acid production. The strain could growth well in the substrates of casin and starch, and however, not in the substrate of tyrosine. It could utilize citrate, too. A sequence alignment of 16s rDNA of the strain HAP was performed and the phylogenetic tree was constructed. The strain was identified as Bacillus alealophilus. The protease activity of strain HAP is 1.22×10^4 U/mL.

关 键 词：嗜碱芽孢杆菌 16S RDNA 分离和鉴定 

分 类 号：Q939.97[生物学—微生物学]