JEG-3细胞分泌孕激素与HLA-G mRNA表达的相关性研究  

Correlations between Progesterone Production and Expression of HLA-G mRNA in JEG-3

作　　者：黄仲英[1] 颜静[2] 

机构地区：[1]四川大学华西第二医院妇产科,四川成都610041 [2]四川大学基础与法医学院,四川成都610041

出　　处：《现代生物医学进展》2008年第7期1270-1271,1279,共3页Progress in Modern Biomedicine

基　　金：日中口川医学奖学金资助

摘　　要：目的:探讨JEG-3细胞自身分泌孕激素与其HLA-G mRNA表达的相关性,为进行滋养叶细胞HLA-G分子表达的调控研究提供线索和依据。方法:培养JEG-3细胞,在不同时点(1,2,3,4,6,12,24h)收集细胞和培养上清液,分别采用实时定量PCR方法和EIA方法测定JEG-3细胞的HLA-G mRNA的表达及孕激素的浓度。结果:JEG-3细胞培养上清液中孕激素浓度与JEG-3细胞HLA-G mRNA表达的强度随时间进程具有相似变化趋势,经Spearman等级相关分析,JEG-3细胞自身孕激素的分泌与其HLA-G mRNA的表达量呈显著正相关(r=0.964,P<0.05)。结论:JEG-3细胞孕激素的分泌与其HLA-G mRNA的表达量具有高度相关性,进一步体外研究孕激素对滋养叶细胞HLA-G分子表达的调节作用时,应考虑到JEG-3细胞自身孕激素分泌的影响。Objective： To investigate the correlations between progesterone production and HLA-G mRNA expressed in JEG-3 cells. Methods： JEG-3 cells were cultured in serum-free medium and cells and culture medium were collected at different time point（ 1, 2, 3, 4, 6,12, 24h）. Real-time PCR was used to quantify the expression of HLA-G mRNA in JEG-3 cell. The level of progesterone concentration in culture medium was measured by EIA. Results： The change tendency of progesterone concentration was similar to that of JHLA-G mR-NA in the time-course experiment. There was significantly positive correlation between progesterone production and cells and HLA-G mRNA expression （r=0.964, P〈0.05 ）. Conclusion： The level of progesterone production in JEG-3 cells is positively correlated with that of HLA-G mRNA in JEG-3 cells. In vitro studies concerning the effect of progesterone on HLA-G expression, the progesterone secreted by JEG-3 itself should be considered.

关 键 词：孕激素 HLA-G JEG-3 滋养叶细胞 

分 类 号：Q132.7[生物学—普通生物学] Q492