甘油对户尘螨过敏原点刺液急性毒性实验的影响  

Influence Glycerin on Mice Acute Toxicity Test of Dermatophagoides Pterronyssinus Skin Prick Test Solution

作　　者：孙劲旅[1] 任华丽[2] 卢朝辉[3] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院变态反应科,北京100730 [2]首都医科大学宣武医院风湿变态科,北京100053 [3]中国医学科学院北京协和医学院北京协和医院病理科,北京100730

出　　处：《中华临床免疫和变态反应杂志》2008年第1期11-13,I0001,共4页Chinese Journal of Allergy & Clinical Immunology

基　　金：国家自然科学基金(30671943);国家863计划(2003AA2Z3502)

摘　　要：摘要:目的探讨甘油对户尘螨过敏原点刺液急性毒性实验的影响。方法按1:10(W/V)比例制备户尘螨过敏原提取液原液。户尘螨过敏原点刺液急性毒性实验:取5～7周龄BALB/c小鼠35只,分成5组,每组7只。在每组小鼠腹腔内分别注射0.5 ml不同液体,具体如下:A组含0.4%苯酚的户尘螨过敏原提取液原液(3 ml)+甘油(3 ml);B组含户尘螨过敏原提取液原液(3 ml)+生理盐水(3 ml);C组含甘油(3 ml)+生理盐水(3 ml);D组含0.4%苯酚的生理盐水(6 ml);E组含生理盐水(6 ml)。结果A组和C组内所有小鼠均在注射后10分钟左右出现活动减少、竖尾,15-18分钟全身躁动、抖动、打滚,20～25分钟小鼠死亡。B、D和E组小鼠腹腔注射后活动正常,观察7天均存活。A组和C组死亡的小鼠立即进行病理解剖,结果显示腹腔内大量腹水、肠管水肿;肺组织高度淤血,肺泡壁增厚增宽,肺泡壁及肺间质毛细血管高度扩张,肺间质可见多量淋巴细胞浸润。小肠壁疏松、水肿,黏膜上皮变性、坏死、脱落。肝、脾、肾组织未见特殊改变。B、D和E组小鼠的肺、肝、脾、肾组织未见特殊改变。结论小鼠户尘螨过敏原点刺液急性毒性实验应该使用不含有50%甘油的户尘螨过敏原点刺液原液。Objective To identify potential influence of glycerin on mouse acute toxicity test of Dermatophagoides Der. pterronyssinus skin prick test （SPT） solution. Methods Stock extract of Der. pterronyssinus SPT solution was prepared in the ratio of 1：10 （W/V） with extract solution without phenol. Stock extract was sterilized with 0.4 μm filters. Thirty five BALB/c mice were divided into 5 groups with abdominal cavity injection of 0.5 ml. Group A： stock extract with 0.4% phenol （3 ml） ＋glycerin （3 ml）; group B： stock extract （3 ml） ＋0.9% sodium chloride injection （NS） （3 ml）; group C： glycerin （3 ml） ＋NS （3 ml）; group D： NS with 0.4% phenol （6 ml）; group E： NS （6 ml）. Autopsy was done for each group. Results All the mice in Group A and C shown decreased activities and their tails cocked up in about 10 minutes after injection. Then, they dithered, and rolled about. All mice in group A and C died within 25 minutes. Autopsy was done immediately, gut edema and ascites could be found. Pulmonary congestion was shown complicated with thickening of alveolar wall, congestion of capillary and lymphocytes infiltration in pulmonary interstitial tissue. Intestinal wall shown edema, mucosa epithelia degeneration, necrosis and excoriation. No abnormal change was shown in liver, spleen and kidney. All mice in Group B, D and E shown normal activities and the biopsy was done after one week. No abnormal phenomena was seen in lung, liver, spleen and kidney. Conclussion Stock extract of Der. pterronyssinus without glycerin should be used in mice acute toxicity test of Der. pterronyssinus SPT solution.

关 键 词：甘油 急性毒性实验 户尘螨 过敏原浸液 

分 类 号：R96[医药卫生—药理学]