胰腺干细胞生物学特性研究进展  

Research progress in the biological characteristics of the pancreatic stem cells

作　　者：效梅[1] 安立龙[1] 窦忠英[2] 

机构地区：[1]广东海洋大学动物医学系,广东湛江524088 [2]西北农林科技大学陕西省干细胞生物工程技术研究中心,陕西杨凌712100

出　　处：《现代生物医学进展》2008年第5期970-972,985,共4页Progress in Modern Biomedicine

基　　金：国家"973"项目(G1999054301);国家"863"项目(2002AA216161);国家自然科学基金(39970363);教育部重点专向;广东省自然科学基金(04011471);广东省教育厅科学基金(2003-1009)

摘　　要：胰腺干细胞是一类来源于胎儿或成年胰腺组织中的成体干细胞。多数研究认为胰腺干细胞体外培养时,贴壁生长,呈多角形上皮样,具有较高的扩增性;表达胰腺发育过程中的一些决定因子Pdx1、HNF3β、Ngn3、Th及Isll等,还共表达胰腺终末细胞释放的激素glucagon、insulin等,甚至共表达来源于其它胚层细胞的表达物CK19、nestin等;具有多分化潜能,其特点是在自然分化过程中,优先分化为胰腺组织细胞,在特定的条件下,也可转分化为其它组织细胞。胰腺干细胞生物学的不断深入研究,为分离、克隆胰腺干细胞及定向诱导其分化为功能性胰岛移植治疗人类糖尿病奠定了基础。Pancreatic stem cells are the adult stem cells derived from the fetal or the adult pancreatic tissues. Most studies have shown that pancreatic stem cells grow adherently, have a polygonal epithelioid morphology and proliferate quickly when cultured in vitro. The stem cells express the Pdxl, HNF3β, Ngn3, TH and Isll which are some crucial factors during pancreatic development, co-express the hormone glucagon and insulin which secreted out from adult pancreatic cell, and even co-express the CK19 and nestin expressed by the cells from the other embryonic layers. Pancreatic stem cells are pluripotent, can prior differentiate into pancreatic cells during the nature differentiation, and can also trans-differentiate into the other cells in the special environment. The study of the biological features of pancreatic stem cells will lay the foundation for farther isolation, cloning and inducing directionally stem cells to differentiate into func- tional islets, and for transplanting the induced islets to reversing human diabetes mellitus.

关 键 词：胰腺干细胞 形态 扩增性 表达特性 分化 

分 类 号：R333.3[医药卫生—人体生理学] Q813[医药卫生—基础医学]