早、中、晚孕期胎盘因子体外抗HIV-1的研究  

A Study on Placental Factors in the First,Second and Third Trimester against HIV-1 in Vitro

作　　者：李莉平[1] 康佳丽[1] 夏薇[1] 曾耀英[2] 

机构地区：[1]广州市第一人民医院妇产科,广东广州510180 [2]暨南大学组织移植与免疫中心,广东广州510632

出　　处：《现代生物医学进展》2008年第6期1001-1004,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金(30500466)~~

摘　　要：目的:探讨早、中、晚孕期胎盘因子(PF)体外抗人免疫缺陷病毒-1(HIV-1)的作用及其在HIV-1垂直传播中可能的作用。方法:采用荧光染料Calcien-AM标记的H9/HIV-1ⅢB分别与早、中、晚孕期不同稀释浓度的PF作用后,与MT2细胞混合培养,荧光显微镜下观察合胞体的形成;用游离的HIV-1ⅢB感染MT2细胞,并分别与早、中、晚孕期不同稀释浓度的PF作用后,用MTT法检测HIV-1感染细胞的存活率,用HIV-1 p24抗原试剂盒检测细胞培养上清中p24抗原含量的变化。结果:各孕期PF并不能抑制MT2和H9/HIV-1ⅢB细胞的融合,但可以增加HIV-1感染细胞的存活率及减少HIV-1 p24抗原的表达,且效应以早孕期PF最大,中孕期PF其次,晚孕期PF最小,并与剂量呈正相关。结论:PF在体外具有抗HIV-1的作用,并呈现孕期和剂量相关性,可能在阻断HIV-1垂直传播中具有一定作用。Objective To systemically investigate the effects of PF in the first, second and third trimesters against HIV-1 infection in vitro, and to evaluate their possible roles in HIV-1 intrauterine infection. Methods Calcein-AM stained H9/HIV-1 Ⅲ B cells were treated with different concentrations of PF in the first, second and third trimesters respectively, which was then mixed with MT2 cells and put under fluorescent microscopy for detection of HIV-1-mediated syncytium formation. MT2 cells were treated with cell-free HIV-1 Ⅲ B, and then washed and cultured with different concentrations of PF in the first, second and third trimesters respectively. Furtherly, the protecting effect of PFs against HIV-l-infected cells was determined with MTT, viral replication was evaluated by measurement of the levels of p24 antigen in culture supematants with ELISA and the inhibition rate was calculated. Results PFs did not suppress HIV-1-mediated syncytiurn formation, however, they significantly increased the viabilities of HIV-1-infected cells and the inhibition rates of p24 antigen levels. Moreover, their effects arrived at the highest in the first trimester, and decreased along with the progress of pregnancy. In addition, the anti-HIV-1 activities of PFs were dose-dependent. Conclusion PFs from placental cells can decrease HIV-1 infection in vitro, and may have an essential role in reducing HIV-1 vertical transmission via placenta.

关 键 词：胎盘因子 HIV-1 垂直传播 Calcien—AM 

分 类 号：Q78[生物学—分子生物学] S852.65[农业科学—基础兽医学]