梓醇对鱼藤酮损伤小鼠的记忆、抗氧化能力的影响及安全性评价  

Protective Effect of Catalpol in Mice Injuries Induced by Rotenone and Evaluation of the Safety of Catalpol

作　　者：姜涛[1] 张爱红[2] 赵荣国 姜波[2] 

机构地区：[1]深圳市宝安区中医院,深圳518133 [2]大连理工大学环境与生命学院,辽宁大连116024 [3]大连国人生物科技有限公司,辽宁大连116600

出　　处：《现代生物医学进展》2008年第6期1039-1041,1045,共4页Progress in Modern Biomedicine

基　　金：辽宁省自然科学基金(20072185)

摘　　要：目的:研究梓醇对鱼藤酮损伤小鼠的学习记忆能力及皮层抗氧化系统的影响,并对其安全性进行评价。方法:腹腔注射鱼藤酮建立小鼠皮层抗氧化系统损伤模型,腹腔注射给药21 d后进行Y迷宫试验,再测定各组小鼠皮层谷胱甘肽过氧化物酶(GSH-PX)、谷胱甘肽S-转移酶(GST)、丙二醛(MDA)、谷胱甘肽(GSH)水平;梓醇安全性评价采取小鼠急性毒性试验和大鼠长期毒性试验。结果:梓醇能够改善小鼠的迷宫成绩,增强GSH-PX活性和GSH含量,降低GST活性和MDA含量,抑制LDH的释放。小鼠腹腔注射给药梓醇的LD50为206.5mg/kg.;大鼠尾静脉注射90天,未见动物出现血液学、血液生化及主要脏器的毒性变化。结论:梓醇能改善鱼藤酮损伤小鼠的记忆能力,减轻皮层氧化应激损伤;长期使用无明显的毒副作用。Objective To investigate the effect of catalpol on spatial learning and memory, and cortex antioxidation system in nerve-injured mice that induced by Rotenone and to evaluate the safety of catalpol. Method Mice were divided into three groups, control group, model group and therapy group. Mice in model group and therapy group were injected intraperitoneally （i.p.） with rotenone for 21 days. Mice in therapy group were injected （i.p.） with catalpol for 10 days, and then they were trained in Y-Maze orderly. Finally the activities of GSH-PX and GST, content of MDA and GSH in the cortex of all the mice were assayed. Acute toxicity and long - term toxicity tests were examined as the safety evaluation. Results Catalpol had an obvious effect on the spatial learning memory, could increase the activities of GSH-PX and content of GSH, the level of MDA and GST decreased after injection with catalpol. The LD50 of catalpol in mice was 206.5 mg/kg. Pathomorphological examination showed that there were no serious pathological changes in the tissue and organs. Conclusions Catalpol had obvious effect on the spatial learning and memory in nerve-injured mice that induced by Rotenone, could relieve the cortex oxidative stress injury. No obvious side effect has been observed.

关 键 词：梓醇 神经损伤 记忆能力 安全性评价 

分 类 号：R971[医药卫生—药品]