转染腺相关病毒对骨髓间充质干细胞分化潜能的影响  

Effect of bone mesenchymal stem cells differentiation potential infected by adeno-associated virus

作　　者：卫静[1] 袁发焕[1] 候卫平[1] 李芙蓉[1] 黄云剑[1] 

机构地区：[1]第三军医大学新桥医院肾内科,重庆400037

出　　处：《现代生物医学进展》2008年第6期1098-1100,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金(30570871);重庆市科委自然科学基金项目(CSTC;2007BB5017)

摘　　要：目的:探讨转染腺相关病毒对骨髓间充质干细胞分化潜能的影响。方法:运用密度梯度离心法分离骨髓问充质干细胞;将pAAV-GFP、pAAV-RC、pHelper用磷酸钙法共转染HEK-293细胞,得到rAAV-GFP,转染骨髓间充质干细胞,进行成骨诱导分化,观察rAAV-GFP对骨髓间充质干细胞分化潜能的影响。结果:与未转染rAAV-GFP的间充质干细胞相比较,转染rAAV-GFP后,骨髓间充质干细胞分化潜能未见改变,均表现为细胞浆蓝紫色,核周明显。结论:转染腺相关病毒对骨髓间充质干细胞分化潜能未见明显影响,为基因修饰腺相关病毒转染骨髓间充质干细胞进行体内移植提供了实验基础。Objective To investigate the effects on potential differentiation of bone marrow mesenchymal stem cells infected by adeno-associated virus. Methods BMSCs were isolated by density gradient centrifugation methods and cultured. The pAAV-GFP was obtained after co-transfetion of HEK-293 by co-precipitation of calcium-phosphate with pAAV-GFP, pAAV-RC and pHelper. BMSCs were infected by the pAAV-GFP, and then were induced. The effects on potential differentiation of bone marrow mesenchymal stem cells infected by adeno-associated virus were studied. Results There was no significant difference between the differentiation propensity of BMSCs infected by rAAV-GFP and BMSCs. The results showed that cytoplasm was blue stained. Conclusions Potential differentiation of bone marrow mesenchymal stem cells isn＇t effected by adeno-associated virus. This research provides an experimental basis for transplantation of bone mesenchymal stem cells infected by adeno-associated virus in vivo.

关 键 词：间充质干细胞 腺相关病毒 分化潜能 

分 类 号：Q78[生物学—分子生物学] Q813