普通小麦-Elymus rectisetus衍生系的细胞学鉴定及其特征特性研究  

Cytogenetic Identification and Characteristics of Triticum aestivum-Elymus rectisetus Derivatives

作　　者：王长有[1] 王秋英[1] 吉万全[1] 薛秀庄[1] 刘雪利[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100

出　　处：《华北农学报》2008年第2期77-79,共3页Acta Agriculturae Boreali-Sinica

基　　金：陕西省重大科技专项(2006Kz05-G3);国家科技攻关项目(2004BA525B03)

摘　　要：利用细胞学方法对2个普通小麦-Elymus rectisetus(Nees in Lehm)A.Lve et Connor衍生系1059A1和1063A1进行了鉴定,并对它们的细胞学稳定性、白粉病抗性、高分子量麦谷蛋白亚基组成和植物学性状进行了研究。2个衍生系在形态学和细胞学上已经基本稳定,根尖体细胞含有44条染色体,花粉母细胞减数分裂中期I(PMC MI)染色体构型为2n=22Ⅱ,与普通小麦亲本Fukuhokomugi杂交F1PMC MI染色体构型为2n=20Ⅱ+3Ⅰ。结果表明,它们均为普通小麦-E.rectisetus异代换-附加系。白粉病抗性鉴定结果表明,2种异代换-附加系高感白粉病。2种异代换-附加系及亲本的SDS-PAGE分析表明,它们的高分子量麦谷蛋白亚基组成与Fukuhokomugi相同。Cytogenetic technique was used to identify two Triticum aestivum-Elymus rectisetus （Nees in Lehm）A. Loeve et Connor derivatives. Additionally, their cytogenetic stability, resistance to powdery mildew, high-molecular-weight subunit of glutenin（HMW-GS）,and morphologic were studied. Chromosome numbers in the root tips and chromosome configuration at PMC MI of two derivatives were 2n = 44 and 2n = 22 Ⅱ. Chromosome configurations at PMC MI of hybrid F1 between two derivatives and Fukuhokomugi were 2n = 20 Ⅱ ＋ 3 Ⅰ. The results showed that two derivatives were Triticum aestivum-Elymus rectisetus alien substitution-addition lines. It was found that E. rectisetus accession 1050 was highly resistant to powdery mildew, and two alien substitution-addition lines and Fukuhokomugi were highly susceptible. Two lines was the same HMWGS as Fukuhokomugi.

关 键 词：披碱草 细胞学 异代换-附加系 高分子量麦谷蛋白亚基 

分 类 号：S512.01[农业科学—作物学] S334.03