芽孢杆菌B96-II对芦笋茎枯病的防治及机制研究  

Causal Study on Inhibition Effects of Antagonistic Bacillus Strain B96-II to Asparagus Stem Blight

作　　者：马利平[1] 郝变青[1] 秦曙[1] 乔雄梧[1] 

机构地区：[1]山西省农业科学院山西省农药重点实验室,山西太原030031

出　　处：《华北农学报》2008年第2期180-184,共5页Acta Agriculturae Boreali-Sinica

基　　金：山西省科技攻关项目(2006031033);山西省重点实验室开放基金项目(2006年)

摘　　要：试验表明,芽孢杆菌B96-II对芦笋茎枯病菌(Phomopsis asparagiSacc)菌丝生长的抑制率为89.62%,对孢子产生的抑制率为99.65%;对芦笋茎枯菌的田间防治效果为93.10%。研究表明,B96-II的拮抗蛋白对芦笋茎枯菌有明显的抑制活性;对芦笋的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)均有激活和诱导作用。B96-II处理后30 d,芦笋组织的SOD活力较对照提高254.98%,CAT活力提高81.84%,POD活性提高22.13%,PPO活力提高146.67%,PAL活力提高171.60%。Antagonistic Bacillus strain B96-II could effectively inhibite mycelia growth and spore germination of Phomopsis asparagi Sacc, pathogen of asparagus stem blight. The inhibition rates were 89.62 % and 99.65 % respectively.Field trials showed a control efficacy of 93.10%. Contents of 5 resistance related enzymes in asparagus plants were stimulated distinctly 30 d after treatment with B96-II. Compared with untreated control sample, superoxide dismutase activity had an increase of 254.98 %, such as 81.84 % to catalase, 22.13 % to peroxidase, 146.67 % to polyphenoloxidase, and 171.60 % to phenylalanin ammonialyase.

关 键 词：芦笋茎枯病 芽胞杆菌 拮抗蛋白 诱导抗性 保护酶 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治]