深圳市志贺菌脉冲场电泳分子分型分析  

Analysis of pulsed-field gel electrophoresis molecular subtyping of Shigella strains in Shenzhen

作　　者：兰全学[1] 扈庆华[1] 石晓路[1] 王冰[1] 林一曼[1] 程锦泉[1] 张顺祥[1] 

机构地区：[1]深圳市疾病预防控制中心,518020

出　　处：《中华预防医学杂志》2008年第5期317-320,共4页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金资助项目（30300281）;广东省卫生厅资助项目（A2003709）

摘　　要：目的 分析深圳市2001—2006年分离志贺菌菌株之间的相关性，初步建立志贺菌的脉冲场电泳（pulsed-field gel eleetrophoresis，PFGE）分子分型监测网络。方法55株志贺菌基因组DNA经Xba I酶切，通过PFGE获得电泳图谱，利用BioNumerics软件对图谱进行聚类分析。结果55株志贺菌被分为41种PFGE图谱，聚类分析显示32株细菌谱型属于同一个群，其余菌株谱型差异较大。结论深圳地区存在遗传紧密相关的志贺菌流行克隆，也存在遗传不相关克隆。PFGE分子分型崎测网络的建立，有助于细菌性痢疾的主动监测、暴发调查和传染源追踪。Objective To analyze the genetic relations of Shigella isolated from Shenzhen in 2001--2006 and develop primary molecular subtyping surveillance network of Shigella. Methods Chromosomal DNAs from 55 isolated in agarose were digested with the restriction enzyme Xba I , and then were analyzed by pulsed-field gel electrophoresis. Pulsed-field gel electrophoresis （PFGE） patterns were clustered using BioNumerics software. Results All 41 distinctive PFGE patterns were identified among 55 strains. 32 strains belonged to one cluster. Differences were observed in other strains. Conclusion Both genetic-related clones and non-related clones of Shigella existed in Shenzhen. The development of PFGE molecular subtyping surveillance network would contribute to the active surveillance, outbreak investigation and source tracking for Shigellosis.

关 键 词：志贺菌属 电泳 凝胶 脉冲场 细菌分型技术 

分 类 号：R686[医药卫生—骨科学]