婴幼儿血管瘤组织中Caspase-3和survivin的表达及其与细胞凋亡的关系  

Apoptosis and expression of caspase-3 and survivin in Infantile Hemangioma

作　　者：钱江[1] 乔成钢[2] 柳宏[2] 王金穗[2] 连树华[2] 郭应芳[2] 党雅梅[2] 

机构地区：[1]兰州大学临床医学院,兰州甘肃730000 [2]甘肃省人民医院

出　　处：《山东医药》2008年第12期7-9,共3页Shandong Medical Journal

基　　金：甘肃省自然科学基金资助项目(3ZS061-A25-107)

摘　　要：目的探讨凋亡调控基因Caspase-3和survivin在婴幼儿血管瘤组织中的表达及与细胞凋亡的关系。方法采用原位杂交法检测血管瘤及正常皮肤组织中Caspase-3 mRNA的表达;用免疫组化SP法检测survivin;用末端脱氧核苷酸转移酶(TdT)介导的d-UTP生物素缺口末端标记技术(TUNEL)检测凋亡细胞。结果Caspase-3mRNA阳性表达率在增生期和消退期分别48.4%和87.5%,两者相比,P<0.01,增生期与正常皮肤组织表达相比,P>0.05。survivin阳性表达率在增生期和消退期分别77.4%和45.8%,两者相比,P<0.01,消退期与正常皮肤组织相比,P>0.05。Caspase-3 mRNA和survivin蛋白表达呈负相关(r=-0.018,P<0.01)。婴幼儿血管瘤增生期和消退期凋亡指数(AI)分别为30.15±10.62和80.16±6.88。AI与Caspase-3 mRNA的表达呈正相关(r=0.405,P<0.01),AI与survivin的表达呈负相关(r=-0.362,P<0.01)。结论Caspase-3基因与凋亡抑制基因survivin参与了血管瘤中细胞凋亡的调节。Objective To investigate the regulation of gene Caspase-3 and survivin in infantile hemangiomas in different periods and significance of the expression of apoptosis, as well as the relationship with apoptosis. Methods Using in situ hybridization detection Caspase-3 mRNA expression of hemangioma and normal skin tissue. Immunohistochemical stainings for survivin was conducted on formalin-fixed, paraffin-embedded tissues of hemangiomas and normal skin tissue. The apoptotic cells in tissues of hemangioma and normal skin tissue were detected by terminal deoxynucleotide transferasemediated deoxyuridine triphosphate-biotin nick end-labeling （TUNEL）. Results There was a significant difference of Caspase-3 mRNA expression between proliferative and involuting phase of hemangioma （48.4% vs 87.5% ,P 〈 0. 01 ）, However,there was no significant difference of Caspase-3 mRNA expression between proliferative hemangioma and normal skin tissue（ P 〉 0.05 ）. Survivin protein expression rate in the proliferative and involuting phase were 77.4% and 45.8%, the difference between the two was statistically significant （ P 〈 0.01 ）, there was no significant difference in the expression of involuting hemangioma and normal skin tissue （ P 〉 0.05 ）. There was a significant negative correlation between Caspase- 3 mRNA and the expression of survivin. Apoptotic index （AI） in proliferative hemangiomas was lower than that in involuting phase hemangiomas （ P 〈 0. 01 ）. There was a significant positive correlation between AI and the expression of Caspase- 3 mRNA （ r = 0. 405, P 〈 0.01 ）, There was a significant negative correlation between AI and the expression of survivin （ r = - 0. 362, P 〈 0. 01 ）. Conclusions Caspase-3 gene may play an important role in the hemangioma and it may be identified as a new therapeutic target. Inhibition of the Caspase-3＇s activation is the anti-apoptosis mechanism of survivin and play an important role in prognosis.

关 键 词：血管瘤 半胱氨酸天冬氨酸蛋白酶-3 生存素 细胞凋亡 

分 类 号：R732[医药卫生—肿瘤]