大鼠MMSCs异基因移植后的生存和对受体免疫状况的影响  

Survival of allogenetic transplanted MMSCs and its influence on recipient eye in rat

作　　者：吴利安[1] 孙慧敏[1] 李筱荣[1] 

机构地区：[1]天津医科大学眼科中心,300070

出　　处：《眼科研究》2008年第5期355-358,共4页Chinese Ophthalmic Research

摘　　要：目的探讨骨髓间充质干细胞(MMSCs)同种异基因移植后的生存状况和对受体免疫状态的影响。方法SD大鼠20只分为MMSCs移植组10只,培养液对照组10只。体外DiL荧光标记MMSCs,移植后共聚焦荧光显微镜下追踪MMSCs在体内的存活情况,观察受体鼠生长状况和眼部情况,比较两组角膜免疫分子MHC-Ⅱ mRNA的表达差异。结果移植4周后大量的MMSCs存活于角膜缘,结构完整,生长良好。受体鼠全身情况良好,眼局部无排斥反应发生。受体鼠角膜表达MHC-Ⅱ,对照组未表达。结论局部同种异基因移植后4周MMSCs在受体眼大量生存,受体全身和眼局部正常,应用于治疗眼表创伤疾病是可行的。Objective Stem cells compose of different types of cells such as embryonic stem cell and adult stem cell and are gradually used to repairing and reconstruction of multiple tissues and organs. The purpose of present study was to investigate the survival of marrow mesenchymal stem cells（MMSCs） and the immune status of recipient after allogenetic transplantation of MMSCs. Methods The MMSCs from femur and tibia of SD rats were isolated,passaged,amplified and pufitied. Twenty SD rats were divided into MMSCs allogenetic transplantation group and control group. The flow cytometry was utilized to detect the expression of CD molecule. The cell suspension containing 5 × 10^3 cells/μL MMSCs was injected on the nasal and temporal subconjunctiva in the fight eyes of 10 SD rats,and the culture medium was used in a same way in other 10 SD rats as control. The survival of DiL-labled MMSCs were invastigated under the confocal microscope, and recipient growth and ocular manifestations were clinically observed. The expression of MHC-Ⅱ mRNA between two groups were compared at 4 weeks after transplantation. Results Cultured MMSCs showed the positive reaction for CD29, CD44 and CD90 but absent reaction for CD31, CD34 and CD45. Adipose-like cells and bone-like cells were identified by Oil Red staining and Von Kossa staining. A lots of MMSCs labled by DiL were seen under the limbal and subconjunctiva in the 4th week after transplantation. No obvious abnormality was found in the eyes during the clinical observation. MHC-Ⅱ mRNA was expressed in cornea of allograft group compared with the control group. Conclusion After local allogenetic transplantation,MMSCs can survive upto 4 weeks. The good status both the system and eye are remained during the observation. These results indicate that local allogenetic transplantation of MMSCs is feasible.

关 键 词：骨髓间充质干细胞 同种异基因移植 主要组织相容性抗原-Ⅱ 

分 类 号：R392[医药卫生—免疫学]