农杆菌介导的莴笋遗传转化体系初步研究  

Study on genetic transformation system of lettuce mediated by Agrobacterium tumefaciens

作　　者：丁建刚[1] 胡定慧[2] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]绵阳师范学院生命科学与技术学院,四川绵阳621010

出　　处：《广西农业科学》2008年第2期123-126,共4页Guangxi Agricultural Sciences

基　　金：西南科技大学青年教师预研基金项目(ZK053040)

摘　　要：为了研究农杆菌介导的莴笋遗传转化的影响因素,建立莴笋高效遗传转化体系,通过设置不同预培养、共培养时间,探讨其对外植体芽分化率的影响;设置不同农杆菌菌液浸染浓度及浸染时间,探讨其对外植体致死率的影响。试验结果表明,对莴笋外植体预培养2d,外植体芽分化率最高;共培养0、1、2d,外植体芽分化率差别不大;当农杆菌菌液OD600值为0.3、0.5,分别侵染1、3、5min,外植体致死率相差不大。因此,在莴笋遗传转化中,采用外植体预培养2d、共培养2d,农杆菌菌液OD600值为0.5、侵染时间5min的遗传转化体系能提高莴笋的遗传转化率。In order to study the influencing factors on genetic transformation of lettuce mediated by Agrobacteriurn tumefaciens, and set up high efficient transformation system, different co-culture and pre-culture time and A. tumefaciens infected concentrations and time were designed to investigate the bud differentiation rate of explant, and the lethal rate of lettuce explant, respectively. The results showed that when the cotyledon and hypocotyl was pre-cultured for two days, the bud differentiation rate was the highest; when the cotyledon and hypocotyl was co-cultured 0, one and two days, respectively, the difference of bud differentiation rate of explant was not obvious. Moreover, when the infected concentration OD600 of A. tumefaciens was 0.3 and 0.5, and infected time was one, three and five minutes, respectively, the difference of lethal rate of explant was not obvious. Therefore, applying genetic transformation system with two days of pre-culture, two days of co-culture, 0.5 of infected concentration OD600 and five minutes of infected time could increase the genetic transformation efficiency.

关 键 词：莴笋 遗传转化 农杆菌介导 

分 类 号：S636.203.6[农业科学—蔬菜学]