受精液中添加β-ME、BSA和咖啡因对猪卵母细胞体外受精后早期发育的影响  

Effects of beta-mercaptoethanol,BSA and caffeine in in vitro fertilization medium on subsequent embryonic development of pig oocytes

作　　者：许丹娜[1] 田加运[1] 何若钢[2] 莫品方[1] 蓝海恩 黄敏瑞 卢晟盛[1] 卢克焕[1] 

机构地区：[1]广西亚热带生物资源保护利用重点实验室,南宁530004 [2]广西大学动物科学技术学院,南宁530004 [3]广西畜牧研究所,南宁530001

出　　处：《广西农业科学》2008年第2期226-230,共5页Guangxi Agricultural Sciences

基　　金：广西科学研究与技术开发计划项目(桂科攻0330004-14);广西科学基金项目(桂科青0447004)

摘　　要：进行受精液中添加不同浓度的β-ME、BSA和咖啡因对猪卵母细胞体外受精及其早期发育的影响试验。结果表明,在受精液中添加β-ME(2、10、50μmol/L)对猪卵母细胞体外受精后的早期发育无显著促进作用,当添加浓度过高(250μmol/L)时对早期胚胎发育反而不利;在受精液中添加咖啡因(1、2、4mmol/L)对猪卵母细胞体外受精后的早期发育无显著的促进作用;在受精液中添加BSA(0.1%、0.2%、0.4%、0.8%)对猪卵母细胞体外受精后的早期发育有一定的促进作用,并以添加0.4%BSA的效果最好。In this study, the effects of beta-mercaptoethanol （β-ME）, bovine serum albumin （BSA） and caffeine in fertilized semen on the subsequent embryonic development of pig oocytes in vitro were investigated. The results showed that supplying 2, 10, 50μmol/L β-ME in fertilized ,semen had no significant effect on the subsequent embryonic development of pig oocytes after in vitro fertilization, while supplying a relative high eoncentration （250 μmol/L） β-ME had a detrimental effect （p〈0.05）. Moreover, supplying 1, 2 and 4 mmol/L caffeine in fertilization medium had no significant effect on subsequent embryonic development of pig oocytes after in vitro fertilization; adding 0.1%, 0.2%, 0.4% and 0.8 % BSA in fertilized semen was relatively beneficial to subsequent embryonic development of pig oocytes, and the effect of 0.4% BSA was the best.

关 键 词：猪 卵母细胞 体外成熟 体外受精 胚胎培养 

分 类 号：S828.35[农业科学—畜牧学]