广西斑点叉尾鮰爱德华氏菌的分离鉴定  

Isolation and identification of Edwardsiella in channel catfish from Guangxi

作　　者：邓显文[1] 谢芝勋[1] 刘加波[1] 谢丽基[1] 庞耀珊[1] 谢志勤[1] 董建宝[1] 黄伟德 刘远新 

机构地区：[1]广西兽医研究所,南宁530001 [2]钦州市水产技术推广站,广西钦州535000 [3]来宾市水产畜牧局,广西来宾546100

出　　处：《广西农业科学》2008年第2期231-235,共5页Guangxi Agricultural Sciences

基　　金：广西科技攻关项目(桂科攻0428001-2)

摘　　要：在广西2处网箱和2处池塘发病斑点叉尾鮰中分离到4株呈β溶血的革兰氏阴性杆菌,经细菌形态、培养特征、生理生化特性鉴定3株为钻鱼爱德华氏菌、1株为迟钝爱德华氏菌,通过PCR检测、PCR产物核苷酸序列测序及Blast比对,进一步确定3株为钻鱼爱德华氏菌、1株为迟钝爱德华氏菌;致病性试验结果显示,这4株分离菌株具有较强的致病性,能引起斑点叉尾鮰发生爱德华氏菌病;药敏试验结果显示,4株分离菌株对氧氟沙星、恩诺沙星、氟苯尼考、氟哌酸等高度敏感。Four pathogenic bacterial strains which are gram-negative bacillus with β-hemolysis, were isolated from the sick channel catfish of two net-cages and two pond-breeding fish farms in Guangxi. Among them, three stains were Edwardsiella ictalur and one was Edwardsiella tarda based on the morphology of bacteria, cultural performances, physiological and biochemical characteristics, and the same result was determined by PCR diagnosis, sequencing and NCB I Blast. The pathogenicity test results showed that four isolated strains had strong pathogenicity and could introduce Edwardsiella disease. The drug sensitivity results showed that four isolated strains were highly sensitive to ofloxacin, chloramphenicol, florfenicol and norfloxacin, etc.

关 键 词：斑点叉尾鲴 钻鱼爱德华氏菌 迟钝爱德华氏菌 分离 鉴定 

分 类 号：S943[农业科学—水产养殖]