用于禽流感同步检测的压电免疫传感器阵列的研制  

Research on the piezoelectric immunosensor array for the simultaneous determination of avian influenza viruses

作　　者：刘毅新[1] 刘仲明[1] 王弘[2] 孙远明[2] 

机构地区：[1]广州军区广州总医院医学实验科,广州510010 [2]华南农业大学食品学院食品质量与安全实验室,广州510642

出　　处：《免疫学杂志》2008年第3期356-358,共3页Immunological Journal

基　　金：广东省重大科技专项资助(2005A2090101)

摘　　要：目的制作用于禽流感检测的四通道压电蛋白芯片。方法采用聚乙烯亚胺(PEI)粘附、戊二醛(Glu)交联法,将禽流感H5、H7、H9、HI抗原分别固定于石英晶体的银电极表面。结果得到了抗原的最佳固定条件和压电蛋白芯片对阳性、阴性标准血清的响应情况。结论该传感器对阳性标准血清的响应值与阴性标准血清的噪声值之比大于2,可用于定性定量检测。Objective To prepare four-chunnel piezoelectric immunity protein chips for simultaneous determination of avian influenza viruses （AIV）. Methods H5, H7, H9 subtype antigens of avian influenza virus were fixed on silver electrode surface of the quartz crystal by polyethyleneimine adhesion and glutaraldehyde cross-linking. Results The optimum condition of the antigen fixing was found. The response value of piezoelectric immunity protein chips to the positive and negative standard serum was detected. Conclusion The response value of the chips to positive standard serum is 2 times higher than that to negative standard serum, which can be used for qualitative and quantitative detection of AIV.

关 键 词：禽流感 同步检测 压电免疫传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]