高效液相色谱法测定奥美拉唑肠溶胶囊中的奥美拉唑磺酰化物  

Determination of omeprazole sulphone in omeprazole enteric-coated capsules by HPLC

作　　者：林敏[1] 

机构地区：[1]福建省药品检验所,福州350001

出　　处：《海峡药学》2008年第4期30-32,共3页Strait Pharmaceutical Journal

摘　　要：目的建立奥美拉唑肠溶胶囊中奥美拉唑磺酰化物的测定方法。方法色谱柱：Zorbax C8（4．6mm×250mm）；流动相：0．01mol·L^-1。磷酸氢二钠-乙腈（75．25）；流速1．0mL·min^-1；检测波长302nm；进样量20μL。结果奥美拉唑磺酰化物在0．6μg·mL^-1-1．4．ug·mL^-1。范围内呈良好的线性关系（r=0．9998。n=5）。平均回收率102．2％，RSD=1．27％。结论本方法简便，迅速，准确。OBJECTIVE To establish a HPLC method for the determination of omeprazole sulphone in omeprazole enteric-coated capsules. METHODS The analytical column was Zorbax-C8 column （4. 6mm×250mm）. The mobile phase was consisted of 0.01mol· L^-1 dibasic sodium phosphate - acetonitrile （75：25）. The flow rate was 1.0mL·min^-1 with UV detection at 302nm.The injection volume was 20μL. RESULTS There were good linear relationship for omeprazole sulphone within the range of 0.6μg·mL^-1-1.4.ug·mL^-1（r = 0. 9998, n = 5）. The average recovery was 102.2 % with RSD of 1.27 %. CONCLUSION The method is simple, efficient and accurate.

关 键 词：奥美拉唑 肠溶胶囊 奥美拉唑磺酰化物 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]