HPLC法测定复方甲硝唑含漱液中甲硝唑、洗必泰及呋喃西林含量  

Determination of metronidazole,chlorhexidine acetate and furacillin in compound metronidazole gargles by HPLC

作　　者：吴雪玲[1] 丁少波[1] 林颖[1] 吴雪婷[1] 

机构地区：[1]广东东莞市人民医院药剂科,东莞523000

出　　处：《海峡药学》2008年第4期34-36,共3页Strait Pharmaceutical Journal

摘　　要：目的建立复方甲硝唑含漱液中甲硝唑、洗必泰及呋喃西林含量测定的方法。方法色谱柱Liehrospher C18（5μm，4．6×250mm），流动相为乙腈：水（含1％磷酸，用三乙胺调至pH3．8）=（20：80），检测波长263nm，流速1mL·min^-1，柱温：35℃。结果甲硝唑浓度在296、4～691．6μg·mL^-1，浓度与色谱峰面积呈良好线性关系，回归方程为C=41．4063＋6．8445×100A（r=0．9996），平均回收率为100．1％；醋酸洗必泰浓度在75、0～175．0μg·mL^-1浓度与色谱峰面积呈良好线性关系，C=23．4434＋6．9297×100A（r=0．9984），平均回收率为100．5％；呋喃西林浓度在4．32-10．08μg·mL^-1范围内，浓度与色谱峰面积呈良好线性关系，回归方程为分别为：C=0．1340＋1．4811×10^-5A（r=0．9995），平均回收率为99．8％。结论该测定方法分离好、干扰少，准确可靠、操作方便，适于复方甲硝唑含漱液的含量测定。OBJECTIVE To establish an HPLC method for determination of metronidazole and chlorhexidine acetate and furacillin in compound metronidazole gargles. METHODS The extract was injected into Lichrospher C18（5μm, 4.6 × 250mm） column at 350C, the mobile phase was acetonitrile-water （with 1% phosphoric acid, adjust pH to 3.8 with triethylamine） （20：30）, the wavelength detection was at 290 nm, the flow rate was 1mL· min^-1.RESULTS Metronidazole concentration were linear in the rarige of 296.4- 691.6μg· mL^-1（r = 0. 9999）, the average recovery was 100.1 96. Chlorhexidine acetate concentration were linear in the range of 75.0 175.0μg·mL^- 1 （r = 0. 9999）, the average recovery was 100.5 % . Furacillin concentration were linear in the range of 4.32-10.08μg·mL^-1（r=0.9999）, the average recovery was 99.8% .CONCLUSION This method is simple, quick and accurate, and can be used for the determination of metronidazole and chtorhexidine acetate and furacillin in collut.

关 键 词：复方甲硝唑含漱液 甲硝唑 洗必泰 呋喃西林 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]