游动放线菌ATCC 12065生物转化强效免疫抑制剂西罗莫司  

Microbial conversion of sirolimus by Actinoplanes sp.ATCC 12065

作　　者：李夸良[1] 黄捷[1] 程元荣[1] 

机构地区：[1]福建医科大学药学院,福州350004

出　　处：《海峡药学》2008年第4期98-101,共4页Strait Pharmaceutical Journal

摘　　要：游动放线菌ATCC 12065生物转化强效免疫抑制剂西罗莫司后产生与西罗莫司不同的4个未知组分(未知组分1,2,3,4),HPLC保留时间分别为25.41min,34.13min,48.46min和52.24 min(西罗莫司的保留时间为26.62min)。经UV、HPLC及LC-MS分析表明,转化产物为西罗莫司衍生物。未知组分1和未知组分2的分子量为916,比西罗莫司的分子量多2;未知组分3和未知组分4的分子量900,比西罗莫司的分子量少14。A powerful immunosuppressant sirolimus, a mTOR inhibitor, was transformed into four fractions （fractionl, 2, 3 and 4） by Actinoplanes sp.ATCC 12065. The results of HPLC showed the retention time of the four fractions were 25.41min, 34.13min, 48.46min and 52.24min, respectively. All four fractions possessed a UV spectra typical of sirolimus.The ESI mass spectra of fractions 1 and 2 were shown M.W.916, which increased in 2 mass units in comparison with sirolimus, however, fractions 3 and 4 were shown M.W. 900, which reduced 14 mass units. In this paper, the primary results of microbial conversion of sirolimus by Actinoplanes sp. ATCC 12065 had been discussed.

关 键 词：西罗莫司 生物转化 游动放线菌ATCC12065 

分 类 号：R979.5[医药卫生—药品]