甲亢大鼠心肌甲状腺激素受体mRNA的表达  

Experimental studieson the thyroid hormone receptor isoforms mRNA in the hyperthyroid rat myocardium

作　　者：刘春蓉[1] 石峰[2] 李兰英[2] 臧晓怡[2] 刘亚敏[1] 孙云[2] 

机构地区：[1]武警医学院病理学教研室,天津300162 [2]天津医科大学内分泌研究所,天津300070

出　　处：《武警医学院学报》2008年第6期473-476,共4页Acta Academiae Medicinae CPAPF

基　　金：天津市自然科学基金资助项目(033605711);武警医学院院级科研基金项目(WY2006-4)

摘　　要：【目的】探讨正常与甲亢大鼠心肌甲状腺激素受体mRNA表达差异。【方法】将16只实验大鼠随机分为正常对照组和甲亢组两组,每组8只:实时荧光定量PCR(FQ-PCR)方法检测正常对照组与甲亢组大鼠心肌甲状腺激素受体各亚型mRNA表达差异。【结果】与正常大鼠心肌相比,甲亢大鼠心肌甲状腺激素受体α1 mRNA表达上调51%(t=9.17,P<0.01),甲状腺激素受体α2 mRNA表达下调58%(t=10.02,P<0.01),甲状腺激素受体β1 mRNA表达不受影响(t=0.96,P>0.05)。【结论】高甲状腺激素水平对甲状腺激素受体α1、α2、β1mRNA表达影响不一致,甲亢大鼠心肌甲状腺激素受体αmRNA的异常表达变化可能与甲亢心脏病的发病密切相关。[Objective] To study the thyroid hormone receptor isoforms mRNA expression diversities between the euthyroid and hyperthyroid rat myocardium. [Methods] Sixteen Wistar rats were divided randomly into two groups （ n = 8） ： （1） normal control： saline, 1 ml, garage, daily. （2） hyperthyroid group： rats were rendered hyperthyroidism with thyroid tablets, 40 mg, garage, daily. Thyroid hormone receptorα1, α2, β1 mRNA expression diversities were determined by real time fluorescence quantitation PCR 3 weeks later between the euthyroid and hyperthyroid rat myocardium. [ Results] Compared with the euthyroid myocardium, the magnitudes of the increased in TRα1 mRNA were 50% （P 〈 0.01）, the magnitudes of the decreased in TRα2 mRNA were 58% （P 〈 0.01）, TRβ1 mRNA was unaffected （P 〉 0.05） in the hyperthyroid myocardium. TRα1/α2 ratio increased. [ Conclusions] High thyroid hormone level differentially affected the expression of TR isoforms mRNA. TRα mRNA abnormal expression may have close relationship with the pathogenesis of hyperthyroid heart disease.

关 键 词：甲亢心脏病 心肌 甲状腺激素受体 

分 类 号：Q786[生物学—分子生物学]