拟南芥STN7和STN8蛋白激酶的结构预测和功能分析  

STRUCTURE PREDICTION AND FUNCTION ANALYSIS OF STN7 AND STN8 PROTEIN KINASE FROM Arabidopsis thaliana

作　　者：胡源[1] 杜林方[1,2] 蒋佳宏[1] 

机构地区：[1]四川大学生命科学学院,生物资源与生态环境教育部重点实验室,成都610064 [2]四川大学纳米生物医学技术与膜生物学研究所

出　　处：《生物物理学报》2008年第2期99-106,共8页Acta Biophysica Sinica

基　　金：国家自然科学基金项目(30270124;39970068);教育部博士点基金项目(20020610094);教育部新世纪人才支持计划(NCET-04-0861);四川大学985的资助~~

摘　　要：STN7和STN8蛋白激酶分别参与了LHCⅡ蛋白和PSⅡ核心蛋白的磷酸化。作者用折叠识别的方法建立了拟南芥蛋白激酶STN7和STN8核心结构域的三维结构,同时结合其他生物信息学方法对STN7和STN8蛋白的结构和作用机制进行了探讨,选择特异位点合成多肽,并制备抗体。结果表明STN7和STN8蛋白激酶活性区域的电荷和形状互补性决定了两者作用底物蛋白的差异,蛋白印迹结果显示分别制备得到了STN7特异抗体和STN8特异抗体,证实结构预测的正确。It is known that two protein kinase STN7 and STN8 are required for photosystem Ⅱ phosphorylation in Arabiclopsis thaliana. However, the main substrates of STN7 and STN8 are different： LHC Ⅱ phosphorylation is mainly dependent on STN7, whereas phosphorylation of PS Ⅱ core proteins depends on STN8. Here the authors predicted the three dimensional structure of core domain of STN7 and STN8 by using fold recognition method. Combining with other bioinformatics methods, the authors compared their structure and action mechanism. Electric charge and structure difference around the catalytic site may determine substrate specificity of these two kinases. Two anti-peptide antibody were designed and prepared based on the combined data from protein analyses. Western blot analysis with thylakoid membranes of Arabidopsis thaliana showed the polyclonal antibodies against each of the peptides were able to act with the parent protein, respectively. Therefor, this antibody can be used as a probe to detect STN7 or STN8.

关 键 词：光系统Ⅱ 蛋白磷酸化 STN7 STN8 核心结构域 

分 类 号：Q55[生物学—生物化学] Q518.1