纳米氧化铜对大鼠海马神经元I_k电流和PC12细胞活性的影响  

EFFECTS OF NANOCUO ON HIPPOCAMPAL NEURONS AND DIFFERENTIATED PC12 CELLS

作　　者：徐兰举[1] 赵景霞[2] 刘仕昌[1] 张涛[2] Guo-gang REN 杨卓[1] 

机构地区：[1]南开大学医学院,天津300071 [2]南开大学生命科学学院生物活性材料教育部重点实验室,天津300071 [3]Queen Mary College,University of London,London E14 NS,UK

出　　处：《生物物理学报》2008年第2期123-128,共6页Acta Biophysica Sinica

基　　金：国家自然科学基金项目(30640037);天津市应用基础研究基金项目(06YFJMJC09400)~~

摘　　要：用全细胞膜片钳方法研究纳米氧化铜(nano CuO)颗粒对大鼠海马CA1区急性分离神经元延迟整流钾通道电流(Ik)的影响,并用MTT方法研究其对神经生长因子(NGF)诱导分化的PC12细胞活性的影响。结果显示5×10-5g/mLnanoCuO能够显著抑制海马CA1区神经元的Ik,使其失活曲线和对照组相比向左偏移,但对其激活过程无明显影响。MTT方法的实验结果显示不同浓度的nanoCuO(5×10-4、5×10-5、5×10-6g/mL)对NGF诱导分化的PC12细胞均有损伤作用,随着浓度的增加,损伤更加明显,呈量效和时效依赖关系。The effects of nanoparticles of CuO on delayed rectifier potassium current （Ik） were studied in acutely isolated CA1 pyramidal neurons of rat hippocampus by using the whole-cell patch-clamp techniques and the effects on differentiated PC12 cell viability were studied with MTT assay. Nanoparticles of CuO mainly inhibited Ik in the concentration of 5×10^-5 g/mL. Nanoparticles of CuO didn＇t shift the steady-state activation curve of Ik but negatively shift the inactivation curve of Ik. Nanoparticles of CuO （5×10^-4,5×10^-5 and 5×10^-6g/mL） induced differentiated PC12 cells damage with dose-and time-dependent manner.

关 键 词：纳米材料 延迟整流钾通道 海马神经元 PCI2 生物效应 

分 类 号：Q74[生物学—分子生物学]