麻疹IgG抗体EIA检测试剂盒的研制及应用  

Development and application of EIA diagnostic kit for the detection of anti-measles IgG

作　　者：詹曦菁[1] 秦晓勇[2] 石枫[3] 孔健[4] 

机构地区：[1]武警医学院微生物学教研室,天津300162 [2]武警医学院附属医院中医科,天津300162 [3]武警医学院教保处,天津300162 [4]北京生物制品研究所,北京100024

出　　处：《武警医学院学报》2008年第7期576-579,共4页Acta Academiae Medicinae CPAPF

摘　　要：【目的】研制检测麻疹IgG抗体的酶联免疫（EIA）试剂，用于确定人群免疫状况、考核麻疹疫苗接种效果及辅助诊断麻疹病毒的感染。【方法】以经超滤、PEG沉淀和蔗糖密度梯度超速离心纯化后的沪191株麻疹病毒为包被抗原，经试验确定EIA检测的最佳反应条件，最终建立麻疹病毒IgGEIA检测试剂盒，并与血凝抑制实验（HI）及SIGMA麻疹IgGEIA检测试剂盒作比较。【结果】纯化后沪191株麻疹病毒的比活性为775．76HAU／mg，以该纯化抗原制备的EIA检测试剂盒的检测下限为12．5mlU／ml，具有良好的线性（r=0．997）和重复性（CV为4．72％～13．51％）。4℃放置6个月、37℃放置3d，该试剂的灵敏度不变，说明其具有良好的稳定性。与血凝抑制实验（HI）相比，该试剂的灵敏度为99．46％，特异度为81．82％，一致性为97．58％，与SIGMA麻疹IgGEIA诊断试剂盒的一致率为95．12％。【结论】该检测试剂盒可以正确评价人体麻疹免疫状况，为流行病学调查提供了一个可靠、客观、灵敏和快速的手段。[Objective] To develope an enzyme immunoassay （EIA） diagnostic kit in detecting anti-measles IgG. [Methods] S191 measles virus was purified by ultrafiltration, PEG precipitation, density gradient ultracentrifugation and immobilized on polystyrene strip. The anti-measles IgG EIA diagnostic kit was established after determining the optimal reaction conditions by a series of tests. The kit was compared with hemagglutination-inhibitation （HI） test and SIG- MA anti-measles IgG EIA kit. [Results] The specific activities of purified S191 measles virus was 775.76HAU/mg . The sensitivity of the kit using S191 as coating antigen to detect anti-measles IgG was 12.5mIU/ml. The sensitivity, hn- earity （r = 0. 997） and reproducibility （CV： 4.72% - 13.51% ） were excellent, it remained the same quality even deposited at 4 ℃ for 6 months or at 37 ℃ for 3 days. As compared with HI test, the sensitivity was 99.46%, the sped- fieity was 81.82%, and agreement was 97.58%. The agreement with SIGMA anti-measles IgG EIA kit was 95.12 %. [Conclusions] The anti-measles IgG EIA kit can properly assess the condition of population immunization, It offers a credible, objective, sensitive and fast means in epidemiologieal investigation.

关 键 词：酶联免疫(EIA) 沪191株麻疹病毒 麻疹IGG抗体 

分 类 号：R446.62[医药卫生—诊断学]