微流控芯片不互溶相酶催化反应显微拉曼检测  

Monitoring of immiscible phase enzyme-catalyzed process in microfluid chip by Raman microscopy

作　　者：郝苇苇[1] 黎薇[1] 庄金亮[1] 张维[1] 周勇亮[1] 

机构地区：[1]厦门大学化学化工学院化学系化学生物学福建省重点实验室,厦门361005

出　　处：《功能材料与器件学报》2008年第2期512-515,共4页Journal of Functional Materials and Devices

基　　金：国家自然科学基金资助项目(No.20675066);教育部留学回国人员科研启动基金资助项目

摘　　要：生物酶的研究对生命现象本质的揭示具有极其重要的意义。通常生物酶反应的研究是在水溶液体系中进行,与其在生物体内所处的水/有机两相界面微环境不相同。通过微流控芯片可获取稳定的水/有机相界面,并利用显微拉曼光谱仪进行微区扫描实现微流控芯片两相酶反应的检测。定量考察了氰根离子在微流控芯片中水相与苯胺相之间扩散行为,并检测了辣根过氧化物酶催化联苯胺与对甲基苯胺的聚合反应。结果表明,在芯片上,非极性分子联苯胺的酶催化反应产物结构组成较极性分子对甲基苯胺更为复杂,而在普通烧杯中的反应则观察不到这个现象。Enzyme catalyzed process is critical for revealing the virtual life. But normally enzymes were investigated in aqueous solution which quite different from the interface between water and organic molecules in vivo micro -environment. Here enzyme catalyzed reaction was carried out at the stable water/organic interface provided by the microfluidic chip, and Raman microscopy was used to monitor the reaction. The diffusion process of cyanide was investigated quantatively, and the polymerization process of benzidine and p-Toluidin were monitored. It was showed that different products from benzidine could be observed at various position in microfluidic chip, which did not happen in beaker or p - Toluidin system..

关 键 词：非水相酶催化 微流控芯片 显微拉曼光谱 

分 类 号：Q814[生物学—生物工程] O657.37[理学—分析化学]