ACE2基因过表达对糖尿病心肌病大鼠心功能的影响  

Effect of ACE2 over-expression on cardiac function in diabetic cardiomyopathic rats

作　　者：于庆涛[1] 高燕燕[2] 张蕾[3] 戴红艳[3] 杨亚培[3] 董秋立[3] 刘斌[3] 张永欢[3] 董波[3] 

机构地区：[1]山东大学附属省立医院内分泌科,济南250021 [2]青岛大学医学院附属医院内分泌科,山东青岛266100 [3]教育部和卫生部心血管重构和功能研究重点实验室,济南250012

出　　处：《山东大学学报（医学版）》2008年第4期331-334,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助项目(30470701);山东省科技厅自然基金资助项目(03BS037,Y2006068)

摘　　要：目的研究腺病毒为载体的血管紧张素转换酶-2(ACE2)基因转染对大鼠糖尿病心肌病(DCM)的治疗作用。方法采用腹腔注射链脲佐菌素(STZ)建立糖尿病大鼠模型,12周后分别将生理盐水、EGFP基因、ACE2基因注入对照组、EGFP组、ACE2组大鼠心脏。转染后10 d,实时荧光定量PCR比较各组大鼠ACE2mRNA的表达,转染后30d,超声检测各组大鼠的心脏功能,Masson染色测定心肌胶原含量,酶联免疫吸附实验(ELISA)法测定心肌血管紧张素Ⅱ(AngⅡ)的水平。结果ACE2组分别与对照组和EGFP组比较,ACE2mRNA的表达明显升高(P<0.05),心脏收缩和舒张功能明显改善(P<0.05),心肌胶原和AngⅡ含量明显减少(P<0.01)。结论ACE2基因过表达可以改善糖尿病心肌病大鼠的心功能。ObServe To explore the effect of edenoviral gene transfer of the angiotensin converting enzyme-2 （ACE2） on diabetic cardiomyupathic rats. Methods Diabetes was induced by a single intraperitoneal injection of STZ in male Wistar rats. After 12 weeks, the rats were randomly divided into three groups： the control group, the EGFP group and the ACE2 group. Sterile saline, recombinant adonoviral carrying the enhanced green fluorescent protein gene （AdV5. eGFP） and recombinant adonoviral carrying the mouse ACE2 gene （AdV5.ACE2） were respectively injected into the myocardia in different groups. 10 days after gone transfection, myocardial ACE2 mRNA expression was determined. 30 days after gene transfection , left ventricular function, myocardial collagen content and myocantial AngⅡ level were also determined. Results Compared with the control and EGFP groups, ACE2mRNA （P 〈0.05） and cardiac function （P 〈0.05） were significantly increased in the ACE2 group while myocardial collagen content （ P 〈 0.01） and AngⅡ level were significantly decreased in the ACE2 group（ P 〈 0.01）. Over-expression of ACE2 may have therapeutic potentials in improving cardiac function.

关 键 词：糖尿病 肽基二肽酶A 血管紧张素II 基因疗法 

分 类 号：R587.1[医药卫生—内分泌]