RSV感染哮喘小鼠气道炎症及重构与激素抵抗的研究  

Correlation between airway inflammation and asthma marine remodeling and corticosteroid resistance in rats with respiratory syncytial virus infection

作　　者：杨艳娜[1] 陈方方[2] 李洧[1] 王艳[1] 王星光[1] 姜淑娟[1] 

机构地区：[1]山东大学附属省立医院呼吸科,济南250021 [2]山东大学附属千佛山医院呼吸科,济南250014

出　　处：《山东大学学报（医学版）》2008年第4期335-339,共5页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助项目(Z2005C04)

摘　　要：目的观察小鼠哮喘模型感染呼吸道合胞病毒(respiratary syncytial virus,RSV)气道炎症及重构与哮喘治疗激素敏感性之间的联系。方法50只BALB/c小鼠随机分对照组,单纯卵蛋白(OVA)致敏哮喘组(A组)、OVA致敏哮喘+地塞米松(DEX)组(B组)、OVA致敏哮喘+RSV感染组(C组)、OVA致敏哮喘+RSV感染+DEX组(D组),每组10只;酶联免疫吸附法(ELISA)检测白细胞介素-4(IL-4)、白细胞介素-5(IL-5)、转化生长因子β1(TGF-β1)及γ干扰素(IFN-γ)浓度;病理切片行HE染色、Van Gieson氏染色,显微镜下观察气管外周炎性分级和气道胶原沉积情况。结果A、C、D组与对照组比较IL-4I、L-5及TGF-β1浓度升高,气道胶原沉积、炎症浸润加重,差异有统计学意义(P<0.05);D组与C组比较,C组与A组比较,TGF-β1浓度升高,差异有统计学意义(P<0.05),气道炎症及气道胶原沉积加重。结论RSV感染哮喘小鼠可引起明显的Th2细胞炎性反应及气道重构;应用激素干预治疗后,气道炎症及重构进一步加重,表明RSV感染哮喘小鼠对激素治疗敏感性差。Objective To explore the relationship of airway inflammation and the murine model to corticosteroid resistance after respiratory syncytial virus （RSV） infection. Methods 50 BALB/c mice were divided into 5 groups： the control group, the OVA group（group A）, the OVA ＋ Dex group（group B）, the OVA/RSV group（group C）, and the OVA/RSV ＋ Dex group（group D）, 10 mice in each group. Then concentrations of interleukin 4（IL-4）, interleukin 5（IL-5）, interferon-γ（IFN-γ）and transfomfing growth factor-β1 （TGF-β1） were determined by ELISA. Pathological slides were stained with hematoxylin-eosin and Van. Gieson, and the degree of the peribronchial inflammation and airway collage deposition were observed, Results Compared with the control group, concentrations of IL-4, IL-5 and TGF-β1 were significantly increased in groups A, C and D and airway inflammalion and collagen deposition were also aggravated（ P 〈0.05）. Also concentrations of IL-4, IL-5 and TGF-β1 and airway inflammarion and remodeling were gradually increased in the order of groups A, C and D（ P 〈 0.05）. Conclusions RSV infection results in a significant Th2 inflammation and airway remodeling in rats with asthma, and rats are not sensitive to corticosteroid treatment.

关 键 词：哮喘模型 呼吸道合胞病毒 炎症介质 胶原沉积 

分 类 号：R562.2[医药卫生—呼吸系统]