TGFβ_1与MMP9及TIMP1在病毒性心肌炎心肌纤维化中的作用机制及清心Ⅱ号干预作用研究  

Study on Effect of TGFβ_1 MMP9 and Its Inhibitor 1 on Myocardial Fibrosis in Chronic Stage of Viral Myocarditis and Interventional Treatment of QingXinⅡ

作　　者：刘旺[1] 王保奇[1] 程志清[1] 徐百鸿[1] 王娟[1] 

机构地区：[1]浙江中医药大学,浙江杭州310053

出　　处：《中华中医药学刊》2008年第5期1010-1013,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省自然科学基金资助项目(Y207807)

摘　　要：目的:探讨TGFβ1与MMP9及TIMP1在病毒性心肌炎心肌纤维化中的作用机制及清心Ⅱ号干预作用。方法:建立病毒性心肌炎慢性期心肌纤维化模型后小鼠随机分为模型组、卡托普利治疗组、清心Ⅱ号高、中、低治疗组,正常组和模型组给予生理盐水灌胃,治疗组分别给予卡托普利和清心Ⅱ号高中低剂量灌胃。疗程结束后分别采集心脏,应用RT-PCR技术检测CVB3-RNA;免疫组化技术检测TGFβ1、MMP9及TIMP1。结果:造模后MMP9明显增高,TIMP1有下降趋势但不明显,TGFβ1明显增高,应用RT-PCR在心肌中可检测出病毒,清心Ⅱ号干预后MMP9、TGFβ1减低,TIMP1有下降趋势,各治疗组病毒减少。结论:病毒、TGFβ1、MMP9及TIMP1在心肌纤维化形成中具有重要作用,清心Ⅱ号具有抗病毒、抗心肌纤维化作用。病毒性心肌炎慢性期的主要病理特征为心脏间质纤维化,临床病理表现为心脏顺应性下降,僵硬度增加,进而引起舒张功能障碍,可发展为DCM,同时也是顽固性心律失常、慢性心功能不全难以逆转、猝死的重要原因之一。由于病毒的持续存在,心肌不断损伤,最终导致心肌纤维化,但具体机制欠清,本实验从细胞因子角度阐述纤维化形成机理并进一步阐明清心Ⅱ号抗心肌纤维化的机制。Objective： TGFβ1 and MMP9 and TIMP1 in viral myocarditis myocardial fibrosis mechanism and the role of intervention Qingxin Ⅱ . Methods ： Establish mice model of myocardial fibrosis in chronic stage of viral myocarditis, then divide the mice model into model group, captopril group, QingXin Ⅱ high dose, medium dose and low dose group. The normal group and model group are given saline through stomach perfusing ,as well as the treated group are given respectively given captopril and QingXin Ⅱ high,medium and low dose in the same way. After the treatment ,hearts are eollected,CVB3 - RNA are detected by RT- PCR, TGFβ1 MMP9 and TIMP1 are detected by immunohistochemieal tech- nique. Results： After having done the model,MMP9 has increased obviously;T IMP1 has the trend to decrease but not obvious; TGFI31 has increased obviously ;virus can be detected in the myocardium by RT- PCR; after the intervention of QingXin Ⅱ , MMP9 and TGFβ1 has decreased, TIMP1 has the tendency to decrease,virus has reduced in treated group. Conclusion： Virus, TGFβ1 ,MMP9 and TIMP1 have important effects in the formation of myocardial fibrosis; QingXin Ⅱ has the function of antivirus and anti - myocardial fibrosis.

关 键 词：病毒 心肌纤维化 卡托普利 免疫组化 清心Ⅱ号 

分 类 号：R542.21[医药卫生—心血管疾病]