大雪枣与金丝小枣中环磷酸腺苷的含量比较  

Comparison of cAMP Content in Big Snow Jujube and Zizyphus Jujube

作　　者：韩利文[1] 刘可春[1] 党立[1] 王思锋[1] 王雪[1] 侯海荣[1] 

机构地区：[1]山东省科学院生物研究所山东省科学院药物筛选研发平台,山东济南250014

出　　处：《中华中医药学刊》2008年第5期1021-1022,共2页Chinese Archives of Traditional Chinese Medicine

基　　金：山东省科技攻关计划项目(2004JJ2202071)

摘　　要：目的:建立大雪枣中环磷酸腺苷的含量测定方法,并与金丝小枣进行对比。方法:采用DiamonsilTMC18色谱柱(4.6mm×250mm,5μm);流动相为:0.05mol/LKH2PO4-甲醇(90∶10);流速:1mL/min;检测波长:254nm。结果:环磷酸腺苷在0.1～1.0μg范围内呈良好线性关系(r=0.9990),平均回收率为98.04%,RSD为1.79%(n=6)。结论:本方法快捷简便、结果准确可靠,可作为大雪枣中有效成分的质量控制方法,大雪枣中环磷酸腺苷含量与金丝小枣相当,产量更大更适合于开发利用。Objective： To determine and compare the content of cyclic adenosine- 3＇,5＇-monophosphospate（cAMP） in Big Snow Jujube and Zizyphus Jujube by HPLC. Methods： cAMP was separated on Diamonsil^TM C18 （250mm × 4.6 mm,5μm）, the moble phase consisted of 0.05 mol/L KH2 PO4 - methol （90：10） at a flow rate of 1.0 mL/min, the detective wavelength was 254 nm. Results： The average recovery of cAMP was 98.04% （ RSD = 1.79%, n = 6）. Conclusion： The method is reproducible, accuracy, and suitable for the quality evaluation of Big Snow Jujube. Comparative of Zizyphus Jujube, the Big Snow Jujube was more suitable for development and utilization.

关 键 词：大雪枣 金丝小枣 环磷酸腺苷 高效液相法 

分 类 号：R284.2[医药卫生—中药学]