Taqman MGB双探针方法检测慢性乙型肝炎病毒YMDD变异的研究  

Taqman MGB dual-probe detecting YMDD motif mutation of hepatitis B virus in chronic infected patients

作　　者：余忠新[1] 陈占国[2] 

机构地区：[1]温州医学院检验医学院,2002年级浙江温州3250001 [2]温州医学院附属第一医院实验诊断中心,浙江温州3250001

出　　处：《检验医学教育》2007年第4期41-44,共4页

摘　　要：目的:探讨Taqman MGB双探针方法检测慢性乙型肝炎病毒YMDD变异的可靠性。方法:采用荧光定量PCR法检测HBV-DNA含量,Taqman MGB双探针方法和测序方法检测其YMDD变异,以测序方法为参考方法,评价Taqman MGB双探针方法的可靠性。结果:246例HBV DNA阳性患者血清标本中,TaqmanMGB双探针方法阳性检出率100%,100例正常对照全部为阴性;YMDD无突变106例,YMDD有突变(YIDD变异+YVDD变异)140例,与核酸测序方法比较,符合率100%。该方法经过敏感度实验,最低检出下限为1×103copies/ml。结论:Taqman MGB双探针方法能便捷、灵敏、特异地检测YMDD基序变异情况,适用于临床检测慢性乙型肝炎患者拉米夫定治疗的YMDD基序变异。Objective： To evaluate the accuracy of Taqman MGB dual-probe detecting YMDD motif mutation of hepatitis B virus in chronic infected patients. Methods：The concentrations of serum HBV DNA were measured by fluorometric real-time PCR. YMDD motif mutations of HBV were detected by Taqman MGB dual-probe and sequence analysis respectively. Sequence analysis test was regarded as reference method. Results： Among the 246 cases serum samples, all were positive for HBV-DNA by Taqman MGB dual-probe, while those in 100 healthy people were negative. 106 cases were wildtype of YMDD motif mutations, 140 cases of mutants with YIDD and YVDD. It was competely accordant with the results by sequence analysis. The sensitivity of Taqman MGB dual-probe is 1 × 10^3 copies/ml. Conclusion ：Taqman MGB dual probe has the superiority of high sensitivity, high specifity, convenience and quickness. It is reliable to monitor YMDD motif mutation for chronic hepatitis B infected patients treated with Lamivudine.

关 键 词：乙型肝炎病毒 变异 拉米夫定 YMDD 

分 类 号：R512[医药卫生—内科学] R512.62[医药卫生—临床医学]