青蛙骨骼染色透明标本的制作及应用  

PRODUCTION AND APPLICATION OF THE DYEING TRANSPARENT SPECIMEN OF FROG'S SKELETON

作　　者：刘万胜[1] 黄明辉[1] 刘力华[1] 郑登秀[1] 

机构地区：[1]湖南永州职业技术学院,永州425006

出　　处：《现代预防医学》2008年第9期1700-1701,共2页Modern Preventive Medicine

摘　　要：[目的]不通过一般解剖方法直接观察青蛙体内整体骨骼的形态和位置关系。[方法]选活蛙经去内脏(去皮或不去皮)或清洁消化道后用95%酒精或5%～10%福尔马林液固定,然后脱水、脱脂、再脱水和染色。[结果]使用化学药品和方法可以将青蛙骨骼染色制成透明标本,达到一般解剖方法难以观察到的青蛙整体骨骼的位置关系和形态。[结论]本法可广泛应用于实验教学和陈列展览。[Objective] Not through general anatomic methods to observe the relationship between the form and position of frog＇s skeleton. [Methods] Selected a live frog, abscised its innards （flayed or not flayed） or made it fixed with 95% .alcobol or 5%-10% formalin after cleaned the alimentary canal, then dewatered, defatted, dewatered it once more and dyed it. [Results] Using chemicals and methods can dye frog＇s skeleton and make it transparent specimen, then can observe the rela-tionship between the form and position of frog＇s skeleton instead of general anatomic methods.[Conclusion] It should be widely used in experiment, teaching and exposition.

关 键 词：青蛙 透明标本 制作及应用 

分 类 号：R322-33[医药卫生—人体解剖和组织胚胎学]