CD133^+脐血干细胞对大肠癌SW480细胞生长、黏附及迁移的影响  

Effect of CD133^+ stem cells from cord blood on the proliferation,adhesion and migration in human colorectal neoplasm cell line SW480

作　　者：熊汉真 杨敏[1] 杨朝晖[1] 李学农[1] 

机构地区：[1]南方医科大学病理学教研室,广东广州510515

出　　处：《中国癌症杂志》2008年第4期241-244,共4页China Oncology

基　　金：国家自然科学基金(No:30770975);广东省科技计划项目(No:2007B031001001)

摘　　要：背景与目的:转移是恶性肿瘤的最重要的生物学特性,是临床上癌症患者死亡的最主要原因,近年实验显示CD133+造血干细胞对鼠源性肿瘤细胞株具有促转移作用,但在人癌实验层次未见报道。本实验旨在体外探讨CD133+脐血干细胞对人大肠癌细胞株SW480增殖、迁移及黏附的影响。方法:采用密度梯度离心及免疫磁珠分选法分离并培养人CD133+脐血干细胞,将CD133+脐血干细胞与SW480共同趋化培养,用MTT法检测肿瘤细胞的增殖及黏附能力,用Transwell小室法检测肿瘤细胞的迁移能力。结果:CD133+人脐血干细胞能显著促进SW480细胞的生长(P<0.05),能促进肿瘤细胞的形态学改变,即肿瘤细胞的核深染,异型明显,细胞呈多角形,能显著促进SW480细胞的黏附及迁移能力(实验组A490为0.11±0.01,对照组A490为0.05±0.01,P<0.05)。结论:CD133+脐血干细胞可显著促进大肠癌细胞的增殖及侵袭能力,宿主造血干细胞可能对肿瘤转移形成具有重要影响。Background and purpose： The most serious aspect of cancer is the emergence of metastases in organs distant from the primary tumor, since most deaths from cancer are due to metastases. In recent years, a series of studies has been undertaken both in vitro and in vivo, and demonstrated that the CD133^＋ hematopoietic stem cell is effective in increasing mice tumor cell metastases, but the efficacy of CD133^＋ stem cells is not well studied for human cancer. We investigated the effects of CD133^＋ stem cells on the proliferation, adhesion and migration in human colorectal neoplasm cell line SW480. Methods： CD133^＋ cells were separated from fresh cord blood mononuclear cells（MNC） by Ficoll density gradient centrifugation and magnetic activated cell-sorting system（ MACS）. The treatment group was the co-culture of CD133^＋ cells and SW480, the control group used the same culture medium without CD133^＋ cells. The effect of CD133^＋ cells on proliferation in SW480 cell was measured by MTT assay, the migration abilities of SW480 cell were assayed in Transwell cell culture, Cell adhesion assay was carried out in a 96 microplate well precoated with fibronection. Results： CD133^＋ cells could significantly improve the growth ability of SW480 cell, not only the ability of proliferation, but also the morphology. The morphology of the cells was remarkably changed. Irregular nucleus, double nucleus and polymorphic nucleus appeared in the treatment group, and the cells were shaped as polygon-like and leptosomatic. In the cell adhesion assay, A490 of the treatment group was 0.11±0.01, A490 of the control group was 0.05±0.01 （ P 〈 0.05）. Conclusions： Our results indicate that the CD133^＋ cell is effective in increasing tumor cell proliferation and invasion. Hematopoietic stem cell may play an important role in the invasion and metastasis of colorectal neoplasm.

关 键 词：CD133^+细胞 脐血 大肠癌 肿瘤转移 SW480细胞 

分 类 号：R730[医药卫生—肿瘤]