Follistatin Related-gene在小鼠成肌细胞中表达的研究  

Expression in Mouse Myoblast Cell of Follistatin Related-gene

作　　者：杨晓宇[1] 郝文博[1] 董顺义[1] 谷文峰[1] 杨剑杰 胡兰[1] 

机构地区：[1]沈阳农业大学畜牧兽医学院,沈阳110161 [2]鞍山市动物疫病控制中心,辽宁鞍山114000

出　　处：《沈阳农业大学学报》2008年第2期243-246,共4页Journal of Shenyang Agricultural University

摘　　要：利用分子克隆技术,采用RT-PCR扩增follistatin related-gene全长cDNA序列,克隆入真核表达载体pEGFP-N1后测序鉴定,并通过脂质体瞬时转染小鼠成肌细胞,采用RT-PCR和SDS-PAGE方法鉴定FLRG蛋白表达情况。结果表明,成功构建的真核表达载体pEGFP-N1-FLRG能成功转染小鼠成肌细胞,RT-PCR和SDS-PAGE电泳证实pEGFP-N1-FLRG在成肌细胞中表达出FLRG蛋白,为深入研究follistatin related-gene奠定了基础。The full length cDNA of follistatin related-gene was amplified through reverse transfer polymerase chain reaction（RT- PCR）and subcloned into eukaryotic expression vector pEGFP-N1. The recombinant plasmid pEGFP-N1-FLRG was transfected into mouse myoblast cells with liposome. The expression of mRNA and protein encoded by this gene was detected respectively with RT-PCR and SDS-PAGE. The result showed that follistatin related-gene was cloned into pEGFP-N1 successfully, and its expression at mRNA and protein level was detected with RT-PCR and SDS-PAGE. The experiment established the basis for further study on the function of follistatin related-gene.

关 键 词：FLRG 转染 真核表达 

分 类 号：Q55[生物学—生物化学]