PEG胁迫下水稻根外皮层厚壁细胞的解剖学研究  

Anatomical studies on the sclerenchyma on the edge of exodermis in rice roots under PEG-induced drought stress

作　　者：孔妤[1] 王忠[1] 熊飞[1] 顾蕴洁[1] 邓亚萍[1] 

机构地区：[1]扬州大学江苏省作物遗传生理重点实验室,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2008年第1期61-65,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(30270795)

摘　　要：通过PEG模拟干旱胁迫,采用Spurr树脂半薄切片结合显微观察的方法,比较水稻和旱稻根外皮层边缘厚壁细胞的形态差异,研究根外皮层边缘厚壁细胞加厚的规律。结果表明:随PEG浓度的升高,水稻(扬稻6号)根外皮层边缘厚壁细胞的胞壁宽度明显增加;旱稻(中旱3号)对低浓度PEG胁迫不太敏感,当PEG浓度达50g·L-1以上时,根外层厚壁细胞表现出明显加厚的趋势,且随PEG胁迫时间延长,厚壁细胞栓质化程度变高。推测水稻根系在干旱胁迫条件下具有一定的缓冲能力,外皮层边缘厚壁细胞在根外层导水率方面发挥显著的质外体屏障作用,可有效地防止水分丧失,保护根组织免受干旱的伤害。The upland rice and lowland rice were tested by comparing the morphologic differences of the sclerenchymatous cell on the edge of exodermis under the stress of different consistency PEG solutions. This study indicated the rule of cell thickening by Spurr resin semi-thin section under a light microscope in method. The result showed that the cell wall of the sclerenchyma was significantly thickened in conventional lowland rice （Yangdao 6） with the increase of PEG concentration; however, upland rice （Zhonghan 3） was not sensitive to low PEG concentration. The thickening of sclerenchymatous cells obviously became stronger at 50 g · L^-1 and higher concentrations. Besides, suberisation of cell walls increased significantly with the time extension. Thus, the conclusion suggested that the roots of rice had the buffer capacity under drought stress. As apoplastic barriers, the sclerenchymatous cell on the edge of exodermis played an important role in the hydraulic conductivity of the OPR, which could effectively resist the water loss and protect the roots from drought injury.

关 键 词：水稻 根 厚壁细胞 PEG胁迫 质外体屏障 

分 类 号：Q944.54[生物学—植物学] S511[农业科学—作物学]