不同鲨鱼皮胶原蛋白的分离及其特性研究  

Study on the characterization and isolation of collagen from different shark skins

作　　者：吴文惠[1] 张艳[1] 包斌[1] 李燕[1] 

机构地区：[1]上海水产大学食品学院,上海200090

出　　处：《中国海洋药物》2008年第2期22-28,共7页Chinese Journal of Marine Drugs

基　　金：上海市重点学科建设项目;上海市教委重点研究项目资助(T1102;06412)

摘　　要：目的探讨不同鲨鱼皮胶原蛋白的分离方法，并对分离胶原蛋白的部分生化特性进行研究。方法用稀碱溶胀酶解法和缓冲液溶胀分离法分别从水鲨头皮和马鲛鲨皮中分离胶原蛋白，利用聚丙烯酰胺凝胶电泳法（SDS-PAGE）、差示扫描量热仪法（DSC）、园二色谱仪法（CD）对分离胶原蛋白进行纯度及分子大小、变性温度和二级结构的测定。结果鲨鱼皮胶原蛋白回收率为鲜重的2．9％～4．1％；分离胶原蛋白在SDS-PAGE上呈现清晰的3条谱带。相对分子质量分别为205，134，118 KDa；水鲨头皮和马鲛鲨皮胶原蛋白的热变性温度分别为69℃、47℃；两种分离胶原蛋白的二级结构均主要是伊折叠和无规卷曲。不存在α-螺旋。结论采用稀碱溶胀酶解法和缓冲液溶胀分离法均能制备高纯度的胶原蛋白，从水鲨头皮和马鲛鲨皮分离的胶原蛋白相对分子质量和分子构成一致。但提取条件的不同能影响胶原蛋白的热变性温度和二级结构。Objective To investigate the methods of isolating collagen from different shark skins and to study its biochemical properties. Methods The collagen from the Blue shark scalp was extracted and purified by enzymolysis after treatment with diluted caustic alkali, and the collagen from the Spanish mackerel skins was extracted and purified by phosphate buffer method, respectively. Subsequently, the relative molecular mass, thermal denatured temperature and secondary structure of extracted collagen were determined. Results The extracted collagen with a yield of 2.9-4.1% showed three clear bands by SDS-PAGE, and the relative molecular mass were 205,134 and 118KDa. The collagen from Blue shark and Spanish mackerel skins maximal thermal denatured temperature was 69, 47 ℃, respectively. The secondary structures of their collagen were primary with β-sheet and the random structure, without α-alix. Conclusion The purified collagen was also extracted by the method of enzymolysis after treatment with diluted caustic alkali and the method of phosphate buffer. The relative molecular mass and the composition of Blue shark collagen and Spanish mackerel collagen were limited. However, the different extracted conditions could affect the specifically biochemical properties of thermal denatured temperature and secondary structure of collagen.

关 键 词：鲨鱼皮 胶原蛋白 变性温度 二级结构 

分 类 号：Q959.42[生物学—动物学] TQ460[化学工程—制药化工]