龟鹿二仙胶抵抗化疗小鼠骨髓CD_(34)^+细胞凋亡的研究  

STUDY ON RESISTANCE EFFECTS OF GUILU ERXIAN JIAO ON APOPTOSIS OF STEM CD_(34)^+ CELLS IN MICE WITH CHEMOTHERAPY

作　　者：林胜友[1] 沈敏鹤[1] 刘振东[1] 冯正权[1] 徐莉[2] 将福升[2] 吴良村[1] 

机构地区：[1]浙江省中医院,杭州310006 [2]浙江中医药大学生命科学系,杭州310053

出　　处：《中国中医药科技》2008年第3期172-173,共2页Chinese Journal of Traditional Medical Science and Technology

基　　金：浙江省自然基金科研项目No.X205007;浙江省中医药管理局科研基金资助No.2005C023

摘　　要：目的:探讨滋阴益气补阳法(龟鹿二仙胶)对化疗小鼠骨髓CD34+造血干/祖细胞凋亡的影响。方法:小鼠采用腹腔注射环磷酰胺(CTX)造模,分组处理,9天后处死。流式细胞仪检测骨髓CD34+细胞凋亡;RT-PCR法检测其Cyt-C、bcl-2mRNA的表达。结果:CTX组FITC+/PI-细胞比例较盐水组明显升高(P<0.05);而高、中、低3个剂量组及单纯中药组FITC+/PI-细胞比例明显降低,与单纯CTX组比较,差异有显著性(P<0.01)。单纯CTX组Cyt-CmRNA表达较盐水组升高(P<0.05),而中、低2个剂量组及单纯中药组表达较单纯CTX组降低(P<0.05);单纯CTX组bcl-2mRNA表达较盐水组减少;而高、中、低3个剂量组及单纯中药组bcl-2mRNA表达较单纯CTX组明显升高,差异有显著性(P<0.01)。结论:滋阴益气补阳法组方的龟鹿二仙胶能有效抑制化疗小鼠骨髓CD34+造血干/祖细胞凋亡,上调bcl-2mRNA表达可能是其机理之一。Objective: To discuss the effects of guilu erxian jiao based on nourishing yin and tonifying qi and yang on stem CD+34 cellular apoptosis induced by chemotherapy. Methods: Mice after being planted with H22 liver cancer cells were randomly divided into 6 groups,which including three dose guilu erxian jiao groups(ip CTX and ig Chinese herbs),CTX,only Chinese herbs and saline control groups. Bone marrow was taken after being given medicine for 9 days. The apoptosis of hematopoietic stem cells(CD34+) was detected by Annexin V/PI FCM,the expression of Cyt-CmRNA and bcl-2 mRNA were detected by RT-PCR. Results: The FITC+/PI-cell proportion of CD34+ cells in CTX group was significant higher than that of saline group,that was lowered in the three dose guilu erxian jiao groups,there was obvious difference between them and CTX group. The expression of Cyt-CmRNA in CD34+ cells in CTX group was significantly higher than that of saline control,Conclusion: Guilu erxian jiao based on nourishing yin and tonifying qi and yang could effectively inhibit the apoptosis of stem CD34+ cells of mice with chemotherapy.

关 键 词：化疗后骨髓抑制 中医药疗法 @龟鹿二仙胶 治疗应用 小鼠 

分 类 号：R285[医药卫生—中药学]