转抗虫基因烟草植株对棉铃虫幼虫抗性能力的研究  

ON THE RESISTANCE OF CpTI TRANSGENIC TOBACCO PLANTS TO COTTON BOLL WORM

作　　者：冯平章[1] 王音[1] 曹煜[1] 刘宝兰[1] 朱祯[2] 李向辉[2] 

机构地区：[1]中国农业科学院植物保护研究所,北京100094 [2]中国科学院遗传研究所

出　　处：《植物保护学报》1997年第4期331-335,共5页Journal of Plant Protection

基　　金：国家"863"计划资助项目

摘　　要：作者等对转CpTI基因烟草植株进行了抗棉铃虫能力的评估。用死亡率、蜕皮指数、平均体重、生物总量和植株受害程度等5个指标,综合判断转基因植株的抗性水平。结果表明,检测的10株转基因植株中对棉铃虫表现为高抗、中抗的各占4株,低抗的占2株。高抗植株测试虫校正死亡率达90％以上,中抗植株测试虫校正死亡率达75％以上,差异显著,在高抗植株上生长的测试虫蜕皮指数低于标准的30％,总虫重仅为对照的1％,中抗植株上生长的测试虫蜕皮指数是标准的30％～40％,总虫重为对照的3％～7％。综合分析结果表明,高、中抗植株对棉铃虫的抗性明显。The resistance of 10 CpTI transgenic tobacco plants to larvae of cotton boll-worm (CBW) Helicoverpa armigera (Hubner) were appraised according to following 5 indices: the corrective mortality, moulting index, average weight, total biomass and damage level of leaf. The results showed that 4, 4 and 2 of the 10 transgenic plants displayed high, middle and larvae fed on high- and middle-resistant plants were more than 90% and 75%, respectively, significantly higher than that on the check plants. The moulting indices of larvae fed on high- and middle-resistant plants was 30% lower than and 30 % - 40 % as many as the check, and the total biomass values of the two groups of larvae were only 1% and 30% -40% of the check, respectively.

关 键 词：转基因 烟草 抗虫基因 抑制剂 棉铃虫 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]