中国小麦黄花叶病毒(WYMV)分布的RT-PCR鉴定  

IDENTIFICATION OF THE WHEAT YELLOW MOSAIC VIRUS OCCURRING IN CHINA BY RT-PCT

作　　者：李大伟[1] 韩成贵[1] 邢恰明 田兆丰[1] 于嘉林[1] 蔡祝南[1] 刘仪[1] 

机构地区：[1]中国农业大学植物病理学系,中国农业大学农业生物技术国家重点实验室

出　　处：《植物病理学报》1997年第4期303-307,共5页Acta Phytopathologica Sinica

基　　金："863"计划资助

摘　　要：利用小麦黄花叶病毒（Ｗｈｅａｔｙｅｌｌｏｗｍｏｓａｉｃｖｉｒｕｓ，ＷＹＭＶ）和小麦梭条花叶病毒（ｗｈｅａｔｓｐｉｎｄｌｅｓｔｒｅａｋｍｏｓａｉｃｖｉｒｕｓ，ＷＳＳＭＶ）的特异性序列为引物，逆转录－ＰＣＲ方法并配合免疫电镜观察，对采自我国四川陕西、河南、安徽、江苏５省９个县、市的２７份感病田小麦样品进行检测。除２份样品未检测到病毒外，其余２５份样品均为小麦黄花叶病毒所侵染，没有发现小麦梭条花叶病毒，由此表明在中国长江中、下游、淮河及渭河流域严重危害小麦的真菌传线状病毒应主要为小麦黄花叶病毒。With primer pairs flanking to RNA- 1 3, terminal sequences of wheat yellow mosaic viurs ( WYMV ) or wheat spindle streak mosaic virus ( WSSMV ) respectively , reverse transcription-poly- merase chain reaction ( RT-PCR ) was carried out. WYMV was identified in 25 of total 27 diseased wheat samples collected from 9 counties of Sichuan ( s ) , Shannxi ( Sh ) , Henan ( H ) , Anhui ( A ) and Jiang.u (J )province of Chin. , hut no WSSMV was found , By this result , together with observation by immuno-sorbent .electronic microscopy , it is confirmed that the filamentous virus occurring in the areas of mid/down valleys of the Yangtze River is wheat yellow mosaic virus , at least most.

关 键 词：小麦 黄花叶病毒 梭条花叶病毒 分布 RT-PCR 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治] S432.41[农业科学—植物保护]