稻曲病菌白化菌株生物学特性研究  

PATHOGEN OF WHITE FALSE SMUT OF RICE

作　　者：王疏[1] 白元俊[1] 周永力[1] 姚健民 白金铠[1] 

机构地区：[1]辽宁省农业科学院植保所,中国农业科学院作物所,沈阳农业大学植保系

出　　处：《植物病理学报》1997年第4期321-326,共6页Acta Phytopathologica Sinica

摘　　要：新发现的稻曲病菌白化菌株其分生孢子座白色，厚垣孢子表面光滑，大小为３．５－６．０×３．５－６．８μｍ。在麦芽糖－稻芽浸渍液中可产生较多的分生孢子。ｐＨ值为６时生长最好。普通培养基中加入几种供试碳源和氮源均有利于菌落生长，以蔗糖为碳源，浓度为２％或以Ｌ－天门冬酰胺为氮源浓度为０．２％～０．３％时时其菌落生长有促进作用。培养过程中可产生对稻种萌发有抑制作用的毒素，而固体培养时产毒能力高于液体培养。白化菌株回接到水稻上可重新产生白色稻曲球。该菌和稻曲病菌的酯酶同功酶和ＲＡＰＤ扩增谱带明显不同。可见白化菌株具有独立于稻曲病菌的新的分类地位。A new strain of false smut of rice-White False Smut of Rice(WFSR)has white stromata.The chlamydospores have smooth surface with size 3.5-6.0μm×3.5-6.8μm.Culturing in malt sugar-rice but soaking liquid,WFSR can product more conidia.The optimum pH value for it's colonygrowth is pH .Several C-sources and N-sources in the experiment increased the growth of colony onsolid medium or in liqiud,and the hyphae grew best under % sucrose as C-source or 0.2%-0.3%L-Asn as N-source.WFSR produce toxins both in solid and in liquid culture,and toxins producedmore in solid culture than in liquid culture.Inoculating WFSR onto rice,it on formed white smuts.Electrophoresis for esterase-isozyme and DNA's RAPD has remarkably different bands between WF-SR and false sumt of rice,therefore,it is consided that the WFSR should be a new and different falsesmut of rice in classification status.

关 键 词：白化菌株 稻曲病菌 厚垣孢子 水稻 生物学特性 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]