环巴胺对胃癌细胞BGC-823增殖凋亡及Shh、Gli-1基因表达的影响  

Effect of cyclopamine on proliferation,apoptosis of gastric carcinoma cell line BGC-823 and expression of Shh and Gli-1 genes

作　　者：任雪萍[1] 姜藻[1] 刘飞[1] 顾晓怡[1] 高峰[1] 

机构地区：[1]东南大学附属中大医院肿瘤中心,江苏南京210009

出　　处：《东南大学学报（医学版）》2008年第3期188-191,共4页Journal of Southeast University(Medical Science Edition)

摘　　要：目的:探讨环巴胺对胃癌细胞BGC-823增殖凋亡及Shh、Gli-1基因表达的影响。方法:采用MTT法检测环巴胺对BGC-823细胞的生长抑制效应,倒置显微镜及AO/EB荧光显微镜观察细胞凋亡的形态学改变,流式细胞术检测细胞凋亡率,RT-PCR检测Shh、Gli-1 mRNA的表达情况。结果:环巴胺可明显抑制BGC-823细胞的生长,呈时间-剂量依赖性,并出现典型的细胞形态学改变;经32、64、96μmol.L-1的环巴胺作用24 h后的凋亡率依次为12.50%、31.50%、56.10%,明显高于空白对照组的2.10%(均P<0.01);Shh及Gli-1 mRNA均表达,环巴胺可下调Gli-1 mRNA表达,但对Shh mRNA表达无明显影响。结论:环巴胺可以抑制胃癌细胞BGC-823增殖并诱导细胞凋亡,其机制可能与下调Gli-1基因表达有关。Objictive To investigate effect of cyclopamine on proliferation, apoptosis of gastric carcinoma cell line BGC-823 and expression of Shh and Gli-1 genes. Methods MTT assay was applied to evaluate cell proliferation inhibition rate. Inverted and AO/EB fluorescence microscopes were used to observe the morphologic change. Flow cytometric analysis was used to determine the apoptosis rate. RT-PCR was used to measure the expression of Shh and Gli-1 mRNA. Results Cyclopamine inhibited BGC-823 cell growth significantly,which acted in dose-dependent and time-dependent manners. Different morphologic alterations were found. The cell apoptosis rates after treated with 32,64,96μmol·L^-1 cyelopamine for 24 h were 12.50% ,31.50% ,56.10%, respectively, higher than control group（ P 〈 0.01 ）. Shh and Gli-1 mRNA all expressed in BGC-823 cells, cyclopamine could decrease the expression of Gli-1 mRNA, instead of not Shh. Conclusion Cyclopamine inhibits proliferation and induces apoptosis of BGC-823 cells, through down-regulating the expression of Gli-1 gene.

关 键 词：环巴胺 胃癌细胞 细胞凋亡 SHH Gli-1 基因表达 

分 类 号：Q253[生物学—细胞生物学] Q255