苯甲酰丙酮-间氯苯甲酰肼西佛碱及其单氧钒配合物的生物活性研究  

The Effects of n-Benzoylacetone- m-Chloro benzoylhydrazone and Its Complex with Oxovanadium(V) on ATPase Activity

作　　者：曾福礼[1] 赵青[1] 王欣[1] 敬兰花[1] 王卫[1] 谭民裕[1] 

机构地区：[1]兰州大学

出　　处：《兰州大学学报（自然科学版）》1997年第4期85-89,共5页Journal of Lanzhou University(Natural Sciences)

基　　金：甘肃省自然科学基金

摘　　要：扼要报道苯甲酰丙酮—间氯苯甲酰肼西佛碱及其单氧钒配合物的合成及其性质，并就它们对Ｎａ＋，Ｋ＋－ＡＴＰａｓｅ活性的影响作了研究．结果表明，配合物浓度大于０．９４μｍｏｌ／Ｌ时，ＡＴＰａｓｅ活性为一定值，配体却表现出促进酶活性的作用；配体一旦与钒生成配合物，酶活性就被抑制，促进作用明显降低，由抑制ＡＴＰａｓｅ活性的动力学曲线还说明在不同浓度的钒配合物存在下，与浓度不同的ＡＴＰ结合位点均呈非竞争性抑制，而ＡＴＰ的Ｋｍ值保持基本不变．The synthesis and properties of nBenzoylacetonemChloro benzoylhydrazone and Its complex with oxovanadium(V) were reported concisely. Its effects on the activity of (Na+,K+)ATPase were studied as well. The results showed that the activity of ATPase was constant at a fixed value when the concentration of the oxovanadium complex was over 0.94 μmol/L, and the ligand showed a promoting effect on enzyme activity. Once the ligand coordinated with oxovanadium to become complex, the activity of (Na+,K+)ATPase was inhibited and the little promoting of ligand remarkably decreased. According to the dynamic curve of (Na+,K+)ATPase activity inhibited by the oxovanadium complex, different concentrations of oxovanadium complex can incompetently inhibit the bound of coresponding concentration ATP with (Na+,K+)ATPase and the affinity constant of enzyme reaction(Km) kept constant. It indicated that the oxovanadium complex has different binding sites with the substrate ATP on (Na+,K+)ATPase

关 键 词：苯甲酰丙酮 钒 配合物 席夫碱 生物活性 

分 类 号：O625.632[理学—有机化学] O614.511[理学—化学]