常年性和季节性发情绵羊品种HIOMT基因的PCR-SSCP分析  

PCR-SSCP analysis of the HIOMT gene in nonseasonal and seasonal estrous sheep breeds

作　　者：于成江[1] 陈玉林[1] 刘福元[2] 尹君亮[2] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]新疆农垦科学院畜牧兽医研究所,新疆石河子832000

出　　处：《西北农林科技大学学报（自然科学版）》2008年第5期12-16,21,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：新疆生产建设兵团博士基金项目(04BSZJ-8)

摘　　要：【目的】探讨季节性与常年性发情绵羊品种在HIOMT基因序列上的差异,为培育常年性发情绵羊品种提供辅助选择标记(MAS)。【方法】采用基因克隆测序及PCR-SSCP技术,分析小尾寒羊、新疆细毛羊、多浪羊和阿勒泰羊共计179个个体的羟基吲哚-氧-甲基转移酶(HIOMT)的3′非翻译区部分序列及其遗传多样性。【结果】在HIOMT基因的3′非翻译区存在PCR-SSCP多态,共发现AA、BB、CC、AB、AC和BC 6种基因型,在小尾寒羊群体中发现以上6种基因型,在新疆细毛羊群体中发现除CC基因型外的其他5种基因型,在阿勒泰羊群体中发现AA、BB、AB和BC 4种基因型,在多浪羊群体中只发现了AA、CC和AC 3种基因型。与AA基因型相比,BB基因型在第94,95,96和201 bp 4处发生了突变,CC基因型在第94,201和217 bp 3处发生了突变。Hardy-Weinberg平衡检验显示,小尾寒羊和多浪羊群体处于平衡状态,新疆细毛羊和阿勒泰羊群体处于非平衡状态。小尾寒羊、新疆细毛羊和阿勒泰羊群体多态信息含量为高度多态(PIC>0.5),多浪羊群体为中度多态(0.25<PIC<0.5)。经χ2独立性检验后,常年性发情的小尾寒羊和多浪羊与季节性发情的新疆细毛羊和阿勒泰羊群体间基因频率差异极显著(P<0.01)。【结论】HIOMT基因在不同绵羊品种中序列的差异性,可能是造成其季节性和常年性发情的原因之一。[Objective] The gene sequence and polymorphism in Hydroxyindole-O-Methyltransferase （HIOMT） gene of 179 individuals including Xinjiang finewool sheep,Small Tall Han sheep, Aletai sheep and Duolang sheep were analyzed. [Method] The study used gene sequencing and PCR-SSCP to analyze the sequencing of 3runtranslated region of HIOMT gene and its genetic diversity in 179 sheep. [Result] The results indicated that there was polymorphism in 3＇untranslated region of HIOMT gene. AA,BB,CC,AB, AC and BC genotypes were found. The six genotypes were all found in Small Tail Han sheep, five genotypes except for CC were found in Xiniiang finewool sheep, AA, BB, AB and BC genotypes were found in Aletai sheep,AA,CC and AC genotypes were found in Duolang sheep. Sequencing revealed four single nucleotide mutations in the BB genotype compared with the AA genotype, three single nucleotide mutations in the CC genotype compared with the AA genotype. The results of Hardy-Weinberg equilibrium test indicated that Small Tail Han sheep, Duolang sheep reached the equilibrium（P〉0.05）, but Xinjiang finewool sheep,Aletai sheep didn＇t （P〈0.05）. Small Tail Han sheep,Xinjiang finewool sheep, Aletai sheep had high polymorphism information co ntents（PIC〉0. 5）,Duolang sheep had moderate polymorphism information contents （0.25〈PIC〈0. 5）. The HIOMT genotype distributions were significantly different（P〈0.01） between nonseasonal estrous sheep（Small Tail Han sheep,Dolang sheep） and seasonal estrous sheep （Xinjiang finewool sheep, Aletai sheep）. [Conclusion] The sequence difference in different sheep breeds indicates that it is one possible reason leading to nonseasonal or seasonal estrous.

关 键 词：绵羊 发情 HIOMT PCR-SSCP 

分 类 号：S826.2[农业科学—畜牧学]