人脊髓灰质炎病毒感染诱发Vero细胞凋亡的研究  

APOPTOSIS AS A CAUSE OF DEATH IN HUMAN POLIOVIRUS INFECTED VERO CELLS

作　　者：李小锋[1] 彭艳华[1] 齐义鹏[1] 林宏 李昌功[1] 

机构地区：[1]武汉大学生命科学学院

出　　处：《武汉大学学报（自然科学版）》1997年第6期789-792,共4页Journal of Wuhan University(Natural Science Edition)

基　　金：国家教委博士点基金

摘　　要：用人脊髓灰质炎病毒分别感染Ｖｅｒｏ细胞和稳定表达ｐ３５基因的Ｖｅｒｏ３５细胞，经细胞核ＤＡＰＩ染色，细胞ＤＮＡ琼脂糖电泳和ＴＤＴ生物素原位标记等方法证实病毒感染的细胞具有典型的凋亡细胞学特征和生物化学特征．实验结果表明人脊髓灰质炎病毒感染Ｖｅｒｏ细胞诱发的细胞死亡能被昆虫杆状病毒凋亡抑制基因ｐ３５抑制，为ＩＣＥ类蛋白水解酶途径的细胞凋亡．Using DAPI staining, agrose gel electrophoresis and TDT biotin labelled hybirdization in situ to check cell DNA, we studied the cytological and biochemical characteristics of Vero cell death caused by infection of poliovirus. The results showed that this cell death is apoptosis.It was found that Vero cells infected with poliovirus died faster than Vero35 cells,in which the apoptosis inhibiting gene p35 of baculovirus can expess stably.The result suggested that poliovirus could induce ICE like mediated pathway of cell death.

关 键 词：脊髓灰质炎病毒 VERO细胞 细胞凋亡 感染 

分 类 号：R512.4[医药卫生—内科学]