荧光素-番红花红T能量转移体系的研究及其作为核酸荧光探针的应用  

Study on the Fluorescein Safranine T Energy Transfer Systems and Its Application as A Fluorescent Probe for Nucleic Acid

作　　者：张红漫[1] 郭祥群[1] 李芳[1] 赵一兵[1] 许金钩[1] 

机构地区：[1]材料和生命过程分析科学国家教委开放研究实验室厦门大学化学系

出　　处：《厦门大学学报（自然科学版）》1997年第6期891-895,共5页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金

摘　　要：研究了以能量转移作为核酸荧光探针的可能性，提出利用能量转移体系测定ＤＮＡ的新方法．该方法灵敏、快速，重现性好，ＤＮＡ测定的线性范围是０．０５～１０ｍｇ／Ｌ，检测限为０．０４７ｍｇ／ＬＣＴＤＮＡ（ｎ＝１１）．The possibility of energy transfer between FL and ST systems as a fluorescent probe for deoxyribonucleic acid was investigated, and based on the energy transfer systems a novel fluorescence method for the determination of double strands DNA has been presented.This method is sensitive, rapid and reproducible. Linearity is observed in the range 0.05 ～ 10 mg/L CT DNA, the limit of detection is 0.047 mg/L CT DNA ( n =11).

关 键 词：荧光素 番红花红T 能量转移 荧光探针 核酸 

分 类 号：Q520.3[生物学—生物化学]