不明原因发热病人血清中登革病毒核酸检测  

Detection and separate of Dengue virus nucleic acid in blood serum of patients with unknown fever in Guizhou

作　　者：周永兵[1] 左丽[1] 刘伟[1] 谢庭华 何成友 王定明[4] 

机构地区：[1]贵阳医学院免疫学教研室 [2]贵州省独山县疾病预防控制中心 [3]贵州省兴义市疾病预防控制中心 [4]贵州省疾病预防控制中心

出　　处：《中国公共卫生》2008年第5期623-624,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30360101);贵州省高层次人才基金〔2005(11)〕;贵州省科学技术基金〔(2006)2083〕

摘　　要：目的对贵州省独山县、兴义市两地夏秋季不明原因发热病人血清进行登革病毒（DEN）核酸检测,以了解贵州省人群DEN的感染情况。方法2005年6-10月份,收集贵州省独山县、兴义市不明原因发热病人血清356份;用常规碘化钠法提取发热病人血清总RNA,用DEN NS1基因区特异性通用引物进行逆转录-聚合酶链式反应法（RT-PCR）,对部分目的条带进行序列测定,并进行氨基酸序列预测和序列同源性分析。结果贵州省独山县、兴义市两地夏秋季不明发热病人血清中DEN NS1基因区的核酸阳性率为9.83%（35/356）;对其中7份标本（兴义5份,独山2份）进行序列测定,均为DEN-2;根据核酸序列进行同源性分析,证实其与DEN-2-43同源性较高,为97.4%（525/539）,7份标本之间的同源性为97.6%（526/539）。结论贵州省独山县、兴义市两地人群中存在DEN感染。Objective To detect Dengue virus nucleic acid in blood serum of patients with unknown fever in Dushan and Xingyi of Guizhou, China in the summer and autumns. Inquiry into the sourec, the evolution characteristics and the relation between the base change and virulence, lay the foundation for immunogenicity and immunologic protection of Dengue virus. Methods Distill Dengue virus DNA by reverse transcriptase polymerase chain reaction （RT - PCR） and detect target cDNA and inoculate C6/36 cells with the blood serum. Results Positive probability of Dengue virus nucleic acid is 9.8% （35/356） in the blood serum samples and seven samples （five of Dushan and and two of Xingyi） were carried through sequence analy- sis. They are Dengue virus type 2 （DEN- 2） through sequence analysis and phylogenic tree, with the same source of DEN- 2 - 44 and DEN - 2 - 43, and the same with DEN - 2 - M that detected from Aedes albopictus in Guizhou in 2003. Inoculating C6/36 cells with the blood serum, positive probability is 1.6% （6/356） from RT - PCR detection. Conclusion There was Dengue virus infection among the population of Guizhou province, China.

关 键 词：贵州 登革病毒 核酸检测 序列分析 

分 类 号：R512.8[医药卫生—内科学]