钾通道开放剂NS1619对哮喘大鼠肺表面活性物质系统超微结构的影响  

Effects of potassium channel opener NS1619 on ultrastructure of pulmonary surfactant system in asthmatic rats

作　　者：杨雍[1] 刘纪宁[1] 吕汝奇[1] 吕祖铭[1] 

机构地区：[1]绵阳市中心医院急诊科,四川绵阳621000

出　　处：《西部医学》2008年第3期471-473,共3页Medical Journal of West China

摘　　要：目的观察新型钾通道开放剂NS1619对哮喘大鼠肺表面活性物质系统(PSS)超微结构的影响。方法以SD大鼠为实验对象,设立对照组,用卵清蛋白(OVA)诱发方式制成SD大鼠哮喘模型。将实验哮喘模型动物随机分为哮喘组、地塞米松组、NS1619组,分别给于雾化吸入生理盐水、地塞米松、NS1619,采用电子显微镜组织化学方法对肺组织的超微结构进行观察。结果对照组、型肺泡上皮(AT-、AT-)细胞形态正常,AT-细胞中板层体形态、结构、数量基本正常。哮喘组AT-、AT-细胞形态有改变,AT-细胞中板层体排空空泡化明显,肺泡腔内可见脱落的管髓体。NS1619组肺泡腔内可见少量脱落的管髓体,AT-、AT-细胞形态改变及AT-细胞中板层体排空空泡化较哮喘组轻。地塞米松组AT-、AT-细胞形态改变及AT-细胞中板层体排空空泡化亦较哮喘组轻。结论PSS超微结构于哮喘后出现明显异常,应用钾通道开放剂NS1619可减轻哮喘后PSS超微结构的异常变化。Objective To examine the effects of NS1619 on ultrastructure of pulmonary surfactant system in rats of asthmatic models. Methods 40 male SD rats were randomly assigned into two groups： control group（10 rats）, asthmatic model group（30 rats）. The rats of model were hypodermically injected lml immunogen stock suspension into abdominal cavity to be sensitized. Two weeks after sensitization, the rats of model were provoked with ultrasonic atomizing inhalation of 1% OVA suspension, After the model was established successfully, the asthmatic SD rats were randomly assigned into three groups： NS1619 group, dexamethasone group, and asthmatic group. Rats in the control group were treated with isotonic natrium chloride inhalation, twenty minutes once a day for 7days. At the same time NS1619 group was treated by ultrasonic atomizing inhalation of 30μmol/L NS1619; dexamethasone group was treated by ultrasonic atomizing inhalation of 30mg/L dexamethasone and asthmatic group by isotonic natrium chloride inhalation. The ultramicrostructure of lung tissue was observed by electron microscope. Results In control group the cells of tape Ⅰ alveolar epithelial （AT- Ⅰ ）, tape Ⅱ alveolar epithelial （AT- Ⅱ ）were eumorphism, and the figure, structure, quantity of laminated bodies in the cells of AT-Ⅱ were normal on the whole. In asthmatic group the figure and structure of laminated bodies were altered, and vacuolization of laminated bodies was manifested. In NS1619 group and dexamethasone group the degree of vacuolization of laminated bodies was lower than that of asthmatic group. Conclusion NS1619 can lighten dysfunction changes of cells AT- Ⅰ , AT- Ⅱ , and laminated bodies. It is beneficial for treatment of asthma.

关 键 词：哮喘 钾离子通道开放剂 NS1619 超微结构 

分 类 号：R562.25[医药卫生—呼吸系统]