黄芪甲甙对吸烟大鼠气道-defensin 2表达的影响  

Effects of astragaloside Ⅳ on β-defensin 2 expression in rat airway induced by cigarette smoking

作　　者：孙贝贝[1] 文富强[1] 王海霞[1] 陈磊[1] 王洵[1] 李继琼[1] 汪涛[1] 徐丹[1] 

机构地区：[1]四川大学华西医院呼吸内科,四川成都610041

出　　处：《西部医学》2008年第3期482-484,共3页Medical Journal of West China

摘　　要：目的了解熏烟对大鼠肺组织β-防御素2(β-defensin2)表达的影响,黄芪甲甙对β-defensin2表达的调控作用及其可能的机制。方法大鼠接受染毒柜被动吸烟三周,建立熏烟模型。分别给于黄芪甲甙1.2mg/kg·d,2.4mg/kg·d,3.6mg/kg·d灌胃。提取肺组织和支气管肺泡灌洗液,采用免疫组化、RT-PCR等方法检测β-defensin 2的表达水平。结果吸烟可以使气道上皮β-defensin 2表达升高,使BALF中炎细胞总数,中性粒细胞,巨噬细胞,淋巴细胞数增高。黄芪甲甙可以降低吸烟诱导的β-defensin 2的高表达,减少支气管肺泡灌洗液白细胞及中性粒细胞,淋巴细胞的数量。结论黄芪甲甙下调吸烟诱导的气道上皮β-defensin 2的高表达,减轻气道炎症。Objective To study the cigarette smoke-induced changes in the expression of rat beta-defensin 2 （rBD-2）in the airway, and evaluate the modulation effect of astragaloside Ⅳ on the expression of rBD-2. Methods Forty male Wistar rats（200-250g）were randomized into cigarette smoking（CS）group, astragaloside Ⅳ（AS-Ⅳ）group, CS＋AS-Ⅳ group and control group, 1.2mg/kg, 2.4mg/kg, 3. 6mg/kg astragaloside Ⅳ was given by daily gavage respectively before the rats were given passive inhalation of tobacco smoke in CS＋AS-Ⅳ group. AS-Ⅳ group was only treated by equal dose of astragaloside Ⅳ and control group by sodium carboxymethycellulose（SCMC）. Animals were killed and samples of lung and BALF were harvested at 25 days. The mRNA of rBD-2 was detected by reverse transcription polymerase chain reaction（RT-PCR）and the protein levels were analyzed by immunohistochemistry（IHC）. Results The expression of rBD-2 mRNA and protein was higher in CS group compared with control group and AS-Ⅳ group, and the number of inflammatory cells in the BALF was also significantly increased. Conclusion Astragaloside Ⅳ downregulates the high expression of rBD-2 induced by cigarette smoking and decrease inflammation reaction.

关 键 词：Β-防御素 黄芪甙 吸烟 肺部感染 

分 类 号：R285.5[医药卫生—中药学]