脂多糖对人肺泡上皮细胞一氧化氮分泌的影响  

Effects of quercetin on LPS-induced nitric oxide released by cultured human alveolar epithelial cells

作　　者：邱容[1] 何正光[1] 文富强[2] 刘代顺[2] 赵勇[1] 

机构地区：[1]遂宁市人民医院呼吸内科,四川遂宁629000 [2]四川大学华西医院,四川成都610041

出　　处：《西部医学》2008年第3期499-500,共2页Medical Journal of West China

基　　金：国家基金杰出青年基金项目(No.30425007);国家自然基金资助项目(30370627);美国纽约CMB基金

摘　　要：目的探讨脂多糖(Lipopolysaccharide,LPS)诱导人肺泡上皮细胞(A549)一氧化氮(Nitric oxide,NO)分泌的影响。方法对A549给予不同浓度的LPS,分别收集其培养上清液,用硝酸还原法检测NO浓度。结果不同浓度LPS诱导A549,NO分泌量高于未经LPS诱导的对照组,且具有浓度、时间依赖性。结论LPS诱导A549NO的分泌,提示LPS具有诱导肺泡上皮细胞急性炎症和氧化应激的作用。Objective To investigate the effects of quercetin on nitric oxide （NO） in human pulmonary epithelial cells （A549） induced by lipopolysaccharide （LPS）. Methods The supernates of LPS-induced cultured A549 from different intervention groups were collected to measure the concentration of NO by nitrate reductase assay. Results The concentration of NO in supernates of LPS-induced A549 was higher than that in the control group （P〈0. 05） in a dose- and time-dependent manner. The concentration of NO in supernates of quercetin intervention groups at 10, 15μmol/L was lower than that in non-intervention group （P〈0. 01）, but that at 5μmol/L was no difference （P〉 0.05）. Conclusion Quercetin can decrease LPS-induced NO secretions in A549, which suggests that quercetin has anti- inflammatory and anti-oxidant stress properties on A549.

关 键 词：肺泡上皮细胞(A549) 脂多糖 一氧化氮(NO) 

分 类 号：R562[医药卫生—呼吸系统]