宿根福禄考组织培养快速繁殖技术  

Rapid Propagation of Phlox paniculata by Tissure Culture

作　　者：殷丽青[1] 黄卫昌[2] 胡永红[2] 罗祺 王新其[1] 

机构地区：[1]上海市农业科学院林木果树研究所,上海201106 [2]上海植物园,上海200231

出　　处：《上海交通大学学报（农业科学版）》2008年第2期161-164,共4页Journal of Shanghai Jiaotong University(Agricultural Science)

摘　　要：以宿根福禄考茎尖和茎节作外植体进行离体快速繁殖,结果显示:无菌芽诱导的适宜培养基为MS+1.0 mg·L^(-1)BA+ 3%糖+6.5 g·L^(-1)琼脂粉,茎节在该培养基上无菌芽的诱导率为87.9%;丛芽增殖适宜培养基为MS+0.5 mg·L^(-1)BA+3%糖+ 6.5 g·L^(-1)琼脂粉,增殖率为6.8;生根的适宜培养基为1/2 MS+0.3 mg·L^(-1)IBA+0.3 mg·L^(-1)NAA+2%糖+6.5 g·L^(-1)琼脂粉,试管苗能在该培养基上分化出良好的根系,生根率达91.7%。Studies were carried out on in vitro rapid propagation of Phlox panieulata, using shoot tips and stem sections as explants. The results showed the optimal medium for inducing the stem sections was MS ＋ 1.0 mg· L^-1 BA ＋ 30 g·L^-1 Sugar ＋ 6.5 g·L^-1 Agar, the inducing rate of stem sections was 87.9%, the optimal medium for propagation of clumpy buds was MS ＋ 0.5 mg·L^-1 BA ＋ 30 g·L^-1 Sugar ＋ 6.5 g·L^-1 Agar, and the propagation coefficient was 6.8; the buds were cultured in 1/2 MS ＋ 0.3 mg·L^-1 IBA＋ 0.3 mg·L^-1 NAA ＋ 20 g·L^-1 Sugar ＋ 6.5 g·L^-1 Agar for 35 days, and 91.7% of test_tube plantlets rooted well.

关 键 词：宿根福禄考(Phlox paniculata) 组织培养 快速繁殖 

分 类 号：S682.39[农业科学—观赏园艺]