不同倍性菘蓝超氧化物歧化酶的活性变化  

Changes of Superoxide Dismutase Activity in Isatis Indigotica Fort with Different Ploidy

作　　者：陈玉芹[1] 聂姬锋[2] 客绍英[1] 陈桂平[1] 宋丽莎[1] 

机构地区：[1]唐山师范学院生命科学系,河北唐山063000 [2]唐山职业技术学院,河北唐山063000

出　　处：《唐山师范学院学报》2008年第2期44-47,共4页Journal of Tangshan Normal University

基　　金：唐山市科技计划重大项目(06234501A-9)

摘　　要：采用聚丙烯酰胺凝胶电泳,NBT光化还原法,以窄叶二倍体、多倍体菘蓝根为实验材料,对不同倍性菘蓝超氧化物歧化酶(SOD)的活性变化进行分析。结果表明:二倍体、多倍体菘蓝的超氧化物歧化酶同工酶谱带数目相同,均为三条,但谱带间均表现出一定的差异,除了A带,多倍体超氧化物歧化酶谱带比二倍体宽且亮。总的趋势为:多倍体的酶带强于二倍体,说明多倍体的酶活性比二倍体高。NBT光化还原法结果显示多倍体SOD比活性为16.21,二倍体SOD比活性为14.58,这一结果同样说明了多倍体的酶活性比二倍体高。由于超氧化物歧化酶与植物的抗逆性密切相关,是生物保护酶系统中的重要成员,因此在一定程度上可说明多倍体植株比二倍体植株的抗逆性强。The activity of Superoxide Dismutase in Isatis indigotica Fort with differrnt ploidies was analyzed by the methods of polyacrylamide gel electrophoresis technology and NBT-illumination method and using the diploid and polyploidy narrow leaf Isatis indigotica Fort as the experimental materials. The result showed that the number of Superoxide Dismutase isoenzyme bands in polyploidy and diploid Isatis indigotica Fort were identical of three. But the degree of strain was displayed some certain differences. Besides band A, the bands in polyploidy were wider and lighter than it in diploid. In all： the enzyme bands in polyploidy were stronger than it in diploid. And it showed that the enzyme activity of the polyploidy was higher than the diploid. And the result obtained through NBT-illumination method showed the relative activity of SOD in polyploidy was 16.21, and the relative activity of SOD in diploid was 14.58, which proved that the activity of the SOD enzyme in polyploidy was higher than in diploid. Because the Superoxide Dismutase had close connection with the resistance of plant and was an important element in protecting enzyme system, the polyploidy plant were higher resistant than the diploid one in some extent.

关 键 词：菘蓝 超氧化物歧化酶 酶活性 

分 类 号：Q814[生物学—生物工程]